BMRB Entry 27303

Name: 1H, 13C and 15N backbone chemical shift assignments of A. thaliana DREB2A(255-272)
Published: 2018-04-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27303

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N backbone chemical shift assignments of A. thaliana DREB2A(255-272)

Deposition date:

2017-11-07

Original release date:

2018-04-13

Authors:

Staby, Lasse; Skriver, Karen; Kragelund, Birthe

Citation:

Citation: Bugge, Katrine; Staby, Lasse; Kemplen, Katherine; O'Shea, Charlotte; Bendsen, Sidsel; Jensen, Michael; Olsen, Johan; Skriver, Karen; Kragelund, Birthe. "Structure of Radical-induced Cell Death1 hub domain reveals a common aa-scaffold for disorder in transcriptional networks" Structure 26, 734-746 (2018).
PubMed: 29657132

Assembly members:

Assembly members:
DREB2A(255-272), polymer, 19 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DREB2A(255-272): GSSDMFDVDELLRDLNGDD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	55
15N chemical shifts	20
1H chemical shifts	21

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DREB2A	1

Entities:

Entity 1, DREB2A 19 residues - Formula weight is not available

N-terminal glycine from TEV cleavage site

1

GLY

SER

ASP

MET

PHE

ASP

VAL

ASP

GLU

2

LEU

ARG

ASP

LEU

ASN

GLY

ASP

Samples:

sample_1: DREB2A, [U-100% 13C; U-100% 15N], 150 uM; sodium chloride 100 mM; DSS 0.7 mM; D2O 10%; PMSF 0.1 mM; sodium azide 0.02 % w/v

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CCPN_Analysis v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking

NMRDraw v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Mdd_NMR v2.5, Orekhov, Jaravine, Mayzel, Kazimierczuk - processing

TOPSPIN v3.5, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 750 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks