BMRB Entry 27312

Title:
Partial solid-state NMR 1H, 13C, 15N resonance assignments of a perdeuterated back-exchanged seven-transmembrane helical protein Anabaena Sensory Rhodopsin
Deposition date:
2017-11-13
Original release date:
2018-03-29
Authors:
Bolton, David; Brown, Leonid; Ladizhansky, Vladimir
Citation:

Citation: Bolton, David; Brown, Leonid; Ladizhansky, Vladimir. "Partial solid-state NMR 1H, 13C, 15N resonance assignments of a perdeuterated back-exchanged seven-transmembrane helical protein Anabaena Sensory Rhodopsin"  Biomol. NMR Assign. 12, 237-242 (2018).
PubMed: 29572785

Assembly members:

Assembly members:
ASR, polymer, 235 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Anabaena variabilis   Taxonomy ID: 264691   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Anabaena variabilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKJ606

Data sets:
Data typeCount
13C chemical shifts186
15N chemical shifts95
1H chemical shifts95

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASR, chain 11
2ASR, chain 21
3ASR, chain 31

Entities:

Entity 1, ASR, chain 1 235 residues - Formula weight is not available

1   METASNLEUGLUSERLEULEUHISTRPILE
2   TYRVALALAGLYMETTHRILEGLYALALEU
3   HISPHETRPSERLEUSERARGASNPROARG
4   GLYVALPROGLNTYRGLUTYRLEUVALALA
5   METPHEILEPROILETRPSERGLYLEUALA
6   TYRMETALAMETALAILEASPGLNGLYLYS
7   VALGLUALAALAGLYGLNILEALAHISTYR
8   ALAARGTYRILEASPTRPMETVALTHRTHR
9   PROLEULEULEULEUSERLEUSERTRPTHR
10   ALAMETGLNPHEILELYSLYSASPTRPTHR
11   LEUILEGLYPHELEUMETSERTHRGLNILE
12   VALVALILETHRSERGLYLEUILEALAASP
13   LEUSERGLUARGASPTRPVALARGTYRLEU
14   TRPTYRILECYSGLYVALCYSALAPHELEU
15   ILEILELEUTRPGLYILETRPASNPROLEU
16   ARGALALYSTHRARGTHRGLNSERSERGLU
17   LEUALAASNLEUTYRASPLYSLEUVALTHR
18   TYRPHETHRVALLEUTRPILEGLYTYRPRO
19   ILEVALTRPILEILEGLYPROSERGLYPHE
20   GLYTRPILEASNGLNTHRILEASPTHRPHE
21   LEUPHECYSLEULEUPROPHEPHESERLYS
22   VALGLYPHESERPHELEUASPLEUHISGLY
23   LEUARGASNLEUASNASPSERARGGLNHIS
24   HISHISHISHISHIS

Samples:

UCND_ASR: Anabaena Sensory Rhodopsin, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mg

RAP_ASR: Anabaena Sensory Rhodopsin, [U-100% 13C; U-100% 15N, 15% 2H], 1 mg

sample_conditions_1: pH: 9; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D CONHUCND_ASRisotropicsample_conditions_1
3D CANHUCND_ASRisotropicsample_conditions_1
3D (CO)CA(CO)NHUCND_ASRisotropicsample_conditions_1
3D CO(CA)NHUCND_ASRisotropicsample_conditions_1
3D CONHRAP_ASRisotropicsample_conditions_1
3D CANHRAP_ASRisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Keller and Wuthrich - data analysis, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks