BMRB Entry 27316

Title:
NMR assignment of the transmembrane helix of BclxL in phospholipid nanodiscs
Deposition date:
2017-11-24
Original release date:
2018-07-12
Authors:
Raltchev, Kolio; Pipercevic, Joka; Hagn, Franz
Citation:

Citation: Raltchev, Kolio; Pipercevic, Joka; Hagn, Franz. "Structural analysis of natively-folded membrane-anchored proteins obtained by SortaseA-mediated ligation"  .

Assembly members:

Assembly members:
BclxL, polymer, 35 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BclxL: GSGESRKGQERFNRWFLTGM TVAGVVLLGSLFSRK

Data sets:
Data typeCount
13C chemical shifts62
15N chemical shifts26
1H chemical shifts26

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BclxL1

Entities:

Entity 1, BclxL 35 residues - Formula weight is not available

1   GLYSERGLYGLUSERARGLYSGLYGLNGLU
2   ARGPHEASNARGTRPPHELEUTHRGLYMET
3   THRVALALAGLYVALVALLEULEUGLYSER
4   LEUPHESERARGLYS

Samples:

sample_1: BclxL, [U-13C; U-15N; U-2H], 400 mM; DMPC, [U-2H], 15 mM; DPMG, [U-2H], 5 mM; potassium phosphate 20 mM; sodium chloride 50 mM; EDTA 0.5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks