BMRB Entry 27341

Name: Backbone assignment of HSV-1 ICP27 103-155
Published: 2018-09-24

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27341

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of HSV-1 ICP27 103-155

Deposition date:

2017-12-15

Original release date:

2018-09-24

Authors:

Tunnicliffe, Richard; Levy, Colin; Mould, Paul; McKenzie, Edward; Sandri-Goldin, Rozanne; Golovanov, Alexander

Citation:

Citation: Tunnicliffe, Richard; Levy, Colin; Mould, Paul; McKenzie, Edward; Sandri-Goldin, Rozanne; Golovanov, Alexander. "Overlapping motifs on the herpes viral proteins ICP27 and ORF57 mediate interactions with the mRNA export adaptors ALYREF and UIF" Sci. Rep. 8, 15005-15005 (2018).

Assembly members:

Assembly members:
ICP27_103-155, polymer, 57 residues, 5977.83 Da.

Natural source:

Natural source: Common Name: Human alphaherpesvirus 1 Taxonomy ID: 10298 Superkingdom: Viruses Kingdom: not available Genus/species: Simplexvirus Human alphaherpesvirus 1

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ICP27_103-155: GPLGSVWSRLGARRPSCSPE RHGGKVARLQPPPTKAQPAR GGRRGRRRGRGRGGPGA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	147
15N chemical shifts	49
1H chemical shifts	49

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ICP27 103-155	1

Entities:

Entity 1, ICP27 103-155 57 residues - 5977.83 Da.

First 4 residues GPLG are non-native, remaining after HRV3C protease cleavage.

1

GLY

PRO

LEU

GLY

SER

VAL

TRP

SER

ARG

LEU

2

GLY

ALA

ARG

PRO

SER

CYS

SER

PRO

GLU

3

ARG

HIS

GLY

LYS

VAL

ALA

ARG

LEU

GLN

4

PRO

THR

LYS

ALA

GLN

PRO

ALA

ARG

5

GLY

ARG

GLY

ARG

GLY

ARG

6

GLY

ARG

GLY

PRO

GLY

ALA

Samples:

sample_1: ICP27 103-155, [U-99% 13C; U-99% 15N], 0.45 mM; phosphate buffer 20 mM; NaCl 50 mM; L-Arg 50 mM; L-Glu 50 mM; 2-mercaptoethanol 50 mM; EDTA 10 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection, processing

SPARKY v3.115, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

UNP	Q9J0X9
AlphaFold	Q9J0X9