BMRB Entry 27355

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal P4 domain of AHNAK
Deposition date:
2018-01-10
Original release date:
2018-04-18
Authors:
Shishmarev, Dmitry; Sundararaj, Srinivasan; Casarotto, Marco
Citation:

Citation: Sundararaj, Srinivasan; Shishmarev, Dmitry; Lin, Yiechang; Aditya, Shouvik; Casarotto, Marco. "1H, 13C and 15N backbone NMR chemical shift assignments of the C-terminal P4 domain of Ahnak"  Biomol. NMR Assignments 12, 253-257 (2018).
PubMed: 29594929

Assembly members:

Assembly members:
AHNAK, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts94
1H chemical shifts359

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AHNAK1

Entities:

Entity 1, AHNAK 108 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   LEUGLUVALLEUPHEGLNGLYPROGLYSER
3   GLYGLYGLUVALSERLEUGLUGLYGLYLYS
4   VALLYSGLYLYSHISGLYLYSLEULYSPHE
5   GLYTHRPHEGLYGLYLEUGLYSERLYSSER
6   LYSGLYHISTYRGLUVALTHRGLYSERASP
7   ASPGLUTHRGLYLYSLEUGLNGLYSERGLY
8   VALSERLEUALASERLYSLYSSERARGLEU
9   SERSERSERSERSERASNASPSERGLYASN
10   LYSVALGLYILEGLNLEUPROGLUVALGLU
11   LEUSERVALSERTHRLYSLYSGLU

Samples:

sample_1: AHNAK, [U-13C; U-15N], 0.5 ± 0.05 mM; sodium phosphate 50 mM; sodium chloride 150 mM; EDTA 2 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 15N TOCSY-HSQCsample_1isotropicsample_conditions_1
3D 15N NOESY-HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

CCPNMR_Analysis v2.4.2, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks