BMRB Entry 27458

Title:
Backbone and Side-chain Chemical Shift Assignments for MarH
Deposition date:
2018-04-20
Original release date:
2018-06-20
Authors:
Liu, Bin; Hu, Kaifeng
Citation:

Citation: Liu, Bin; Fang, Shiqi; Ma, Xiaofang; Bai, Zhiqiang; Hu, Kaifeng. "Backbone and side-chain chemical shift assignments of MarH, a critical intermediary epimerase for biosynthesis of Maremycins in Streptomyces"  Biomol. NMR Assign. 12, 335-338 (2018).
PubMed: 30054867

Assembly members:

Assembly members:
MarH, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptomyces   Taxonomy ID: 1883   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts489
15N chemical shifts116
1H chemical shifts786

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MarH dimer, subunit 11
2MarH dimer, subunit 21

Entities:

Entity 1, MarH dimer, subunit 1 126 residues - Formula weight is not available

1   GLYSERARGPROALAASPPROGLUILEVAL
2   GLUGLYLEUPROILEPROLEUALAVALALA
3   GLYHISHISGLNPROALAPROPHETYRLEU
4   THRALAASPMETPHEGLYGLYLEUPROVAL
5   GLNLEUALAGLYGLYGLULEUSERTHRLEU
6   VALGLYLYSPROVALALAALAPROHISTHR
7   HISPROVALASPGLULEUTYRLEULEUVAL
8   SERPROASNLYSGLYGLYALAARGILEGLU
9   VALGLNLEUASPGLYARGARGHISGLULEU
10   LEUSERPROALAVALMETARGILEPROALA
11   GLYSERGLUHISCYSPHELEUTHRLEUGLU
12   ALAGLUVALGLYSERTYRCYSPHEGLYILE
13   LEULEUGLYASPARGLEU

Samples:

sample_1: MarH, [U-99% 13C; U-99% 15N], 0.4 mM; Na2HPO4 50 mM; KH2PO4 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks