BMRB Entry 27508

Title:
Backbone resonance assignemnts of a Smurf2 HECT domain C-lobe mutant
Deposition date:
2018-06-06
Original release date:
2018-07-12
Authors:
Jaeckl, Magnus; Stollmaier, Carsten; Stroh ker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona; Wiesner, Silke
Citation:

Citation: Jaeckl, Magnus; Stollmaier, Carsten; Strohaeker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona; Wiesner, Silke. "Beta-Sheet Augmentation Is a Conserved Mechanism of Priming HECT E3 Ligases for Ubiquitin Ligation"  J. Mol. Biol. 430, 3218-3233 (2018).
PubMed: 29964046

Assembly members:

Assembly members:
clobe, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-M30

Data sets:
Data typeCount
13C chemical shifts234
15N chemical shifts113
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1clobe1

Entities:

Entity 1, clobe 122 residues - Formula weight is not available

1   GLYALAMETGLYLYSILEASPVALASNASP
2   TRPLYSVALASNTHRARGLEULYSHISALA
3   THRPROASPSERASNILEVALLYSTRPPHE
4   TRPLYSALAVALGLUPHEPHEASPGLUGLU
5   ARGARGALAARGLEULEUGLNPHEVALTHR
6   GLYSERSERARGVALPROLEUGLNGLYPHE
7   LYSALALEUGLNGLYALAALAGLYPROARG
8   LEUPHETHRILEHISGLNILEASPALAASN
9   THRASNASNLEUPROLYSALAHISTHRCYS
10   PHEASNARGILEASPILEPROPROTYRGLU
11   SERTYRGLULYSLEUTYRGLULYSLEULEU
12   THRALAILEGLUGLUTHRSERGLYPHEALA
13   VALGLU

Samples:

sample_1: clobe, [U-100% 13C; U-100% 15N], 2.1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; D2O, [U-99% 2H], 10 % w/v

sample_conditions_1: ionic strength: 0.2 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks