BMRB Entry 27511

Name: Backbone resonance assignment of a Huwe1 HECT domain C-lobe mutant
Published: 2018-07-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27511

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment of a Huwe1 HECT domain C-lobe mutant

Deposition date:

2018-06-06

Original release date:

2018-07-12

Authors:

Wiesner, Silke; Jaeckl, Magnus; Stollmaier, Carsten; Strohaeker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona

Citation:

Citation: Jaeckl, Magnus; Stollmaier, Carsten; Strohaeker, Timo; Hyz, Karolina; Maspero, Elena; Polo, Simona; Wiesner, Silke. "Beta-Sheet Augmentation Is a Conserved Mechanism of Priming HECT E3 Ligases for Ubiquitin Ligation" J. Mol. Biol. 430, 3218-3233 (2018).
PubMed: 29964046

Assembly members:

Assembly members:
clobe, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-M11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
clobe: GAMGLPTIDIDDLKSNTEYH KYQSNSIQIQWFWRALRSFD QADRAKFLQFVTGTSKVPLQ GFAALEGMNGIQKFQIHRDD RSTDRLPSAHTCFNQLDLPA YESFEKLRHMLLLAIQENSE GFGLA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	235
15N chemical shifts	118
1H chemical shifts	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	clobe	1

Entities:

Entity 1, clobe 124 residues - Formula weight is not available

N-terminal GAMG is a cloning artefact. The construct contains a C378N mutation.

1

GLY

ALA

MET

GLY

LEU

PRO

THR

ILE

ASP

ILE

2

ASP

LEU

LYS

SER

ASN

THR

GLU

TYR

HIS

3

LYS

TYR

GLN

SER

ASN

SER

ILE

GLN

ILE

GLN

4

TRP

PHE

TRP

ARG

ALA

LEU

ARG

SER

PHE

ASP

5

GLN

ALA

ASP

ARG

ALA

LYS

PHE

LEU

GLN

PHE

6

VAL

THR

GLY

THR

SER

LYS

VAL

PRO

LEU

GLN

7

GLY

PHE

ALA

LEU

GLU

GLY

MET

ASN

GLY

8

ILE

GLN

LYS

PHE

GLN

ILE

HIS

ARG

ASP

9

ARG

SER

THR

ASP

ARG

LEU

PRO

SER

ALA

HIS

10

THR

CYS

PHE

ASN

GLN

LEU

ASP

LEU

PRO

ALA

11

TYR

GLU

SER

PHE

GLU

LYS

LEU

ARG

HIS

MET

12

LEU

ALA

ILE

GLN

GLU

ASN

SER

GLU

13

GLY

PHE

GLY

LEU

ALA

Samples:

sample_1: clobe, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; D2O, [U-99% 2H], 10 % w/v

sample_conditions_1: ionic strength: 0.2 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz