BMRB Entry 27532

Title:
Backbone and side chain NMR assignments for the ribosome Binding Factor A (RbfA) from Staphylococcus aureus
Deposition date:
2018-07-03
Original release date:
2018-10-03
Authors:
Usachev, Konstantin; Blokhin, Dmitriy; Bikmullin, Aydar; Nurullina, Liliya; Validov, Shamil; Garaeva, Natalia; Klochkov, Vladimir; Aganov, Albert; Khusainov, Iskander; Yusupov, Marat
Citation:

Citation: Blokhin, Dmitriy; Bikmullin, Aydar; Nurullina, Liliya; Garaeva, Natalia; Validov, Shamil; Klochkov, Vladimir; Aganov, Albert; Khusainov, Iskander; Yusupov, Marat; Usachev, Konstantin. "Backbone and side chain NMR assignments for the ribosome binding factor A (RbfA) from Staphylococcus aureus"  Biomol. NMR Assignments 13, 27-30 (2019).
PubMed: 30225569

Assembly members:

Assembly members:
RbfA, polymer, 118 residues, 13669.7691 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts339
15N chemical shifts102
1H chemical shifts390
coupling constants72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RbfA1

Entities:

Entity 1, RbfA 118 residues - 13669.7691 Da.

1   GLYSERSERMETARGALAGLUARGVALGLY
2   GLUGLNMETLYSLYSGLULEUMETASPILE
3   ILEASNASNLYSVALLYSASPPROARGVAL
4   GLYPHEILETHRILETHRASPVALVALLEU
5   THRASNASPLEUSERGLNALALYSVALPHE
6   LEUTHRVALLEUGLYASNASPLYSGLUVAL
7   GLUASNTHRPHELYSALALEUASPLYSALA
8   LYSGLYPHEILELYSSERGLULEUGLYSER
9   ARGMETARGLEUARGILEMETPROGLULEU
10   METTYRGLUTYRASPGLNSERILEGLUTYR
11   GLYASNLYSILEGLUARGMETILEGLNASP
12   LEUHISLYSGLNASPARGVALGLU

Samples:

sample_1: RbfA, [U-100% 13C; U-100% 15N], 0.9 mM; phosphate buffer 50 mM; NH4Cl 250 mM

CondSet1: ionic strength: 0.250 M; pH: 7.600; pressure: 1.000 atm; temperature: 308.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicCondSet1
2D 1H-13C HSQC/HMQCsample_1isotropicCondSet1
2D 1H-13C HSQC/HMQCsample_1isotropicCondSet1
3D HNCAsample_1isotropicCondSet1
3D HNCACBsample_1isotropicCondSet1
3D CBCA(CO)NHsample_1isotropicCondSet1
3D HNCOsample_1isotropicCondSet1
HN(CA)CO (H[N[ca[CO]]])sample_1isotropicCondSet1
CC(CO)NH (HNco_C.relayed)sample_1isotropicCondSet1
3D H(CCO)NHsample_1isotropicCondSet1
3D HNHAsample_1isotropicCondSet1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks