BMRB Entry 27674

Title:
Chemical shifts for C-tail of the apelin receptor in LPPG micelles.
Deposition date:
2018-11-02
Original release date:
2018-11-09
Authors:
Rainey, Jan; Pandey, Aditya
Citation:

Citation: Pandey, Aditya; LeBlanc, Danielle; Parmar, Hirendrasinh; Pham, Tran Thanh Tam; Sarker, Muzaddid; Xu, Lingling; Duncan, Roy; Liu, Xiang-Qin; Rainey, Jan. "Structure, amphipathy, and topology of the membrane-proximal helix 8 influence apelin receptor plasma membrane localization"  Biochim. Biophys. Acta Biomembr. 1861, 183036-183036 (2019).
PubMed: 31394100

Assembly members:

Assembly members:
AR_ctail, polymer, 71 residues, 7488.1419 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETHN

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts146
15N chemical shifts45
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AR ctail1

Entities:

Entity 1, AR ctail 71 residues - 7488.1419 Da.

1   ALAPHEPHEASPPROARGPHEARGGLNALA
2   SERTHRSERMETLEUCYSCYSGLYGLNSER
3   ARGSERALAGLYTHRSERHISSERSERSER
4   GLYGLULYSSERALASERTYRSERSERGLY
5   HISSERGLNGLYPROGLYPROASNMETGLY
6   LYSGLYGLYGLUGLNMETHISGLULYSSER
7   ILEPROTYRSERGLNGLUTHRLEUVALVAL
8   ASP

Samples:

sample_1: AR ctail, [U-13C; U-15N], 0.5 mM; DSS 1 mM; DTT 10 mM; sodium azide 1 mM; sodium chloride 100 mM; sodium phosphate 20 mM; LPPG 100 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 310.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
HN(CA)CO (H[N[ca[CO]]])sample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
CBCACONH (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.2.2, CCPN - data analysis

DANGLE v1.1, CCPN - data analysis

NMRPipe v8.1, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Topspin v3.1, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks