BMRB Entry 30070

Title:
The NMR Solution Structure of RPA3313
Deposition date:
2016-04-29
Original release date:
2016-06-09
Authors:
Catazaro, Jonathan; Lowe, Austin; Powers, Robert; Structural Genomics Consortium (SGC), SGC
Citation:

Citation: Catazaro, Jonathan; Lowe, Austin; Cerny, Ronald; Powers, Robert. "The NMR Solution Structure and Function of RPA3313: A Hypothetical Protein from R. palustris"  Proteins 85, 93-102 (2017).
PubMed: 27802574

Assembly members:

Assembly members:
Uncharacterized protein, polymer, 91 residues, 9887.637 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 258594   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Rhodopseudomonas Rhodopseudomonas palustris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts246
15N chemical shifts68
1H chemical shifts422

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 91 residues - 9887.637 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYMETGLYLYSALATYRTYRASPILEVAL
4   GLYSERASPASNARGTRPGLYILEARGHIS
5   ASPASPASPPROTHRGLYASPTYRSERSER
6   LYSGLUALAALAPHEGLUALAALACYSALA
7   ALAALASERASNALAILELYSPHEGLYHIS
8   GLUVALARGILETHRVALPROGLYASNASP
9   GLYSERGLUTHRASNLEUGLYVALILETHR
10   ASN

Samples:

sample_1: RPA3313, none, 1 mM; MES 18 mM; sodium azide 0.1%; sodium chloride 80 mM

sample_conditions_1: ionic strength: 80 mM; pH: 5.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CC(CA)NHsample_1isotropicsample_conditions_1
3D HCC(CO)NHsample_1isotropicsample_conditions_1
3D CC(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment, peak picking

X-PLOR NIH v2.41.1, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks