BMRB Entry 30339

Title:
Solution structure of Magi3 a specific insect toxin from the spider Macrothele gigas
Deposition date:
2017-09-06
Original release date:
2018-01-05
Authors:
del Rio Portilla, F.; Titaux Delgado, G.; Escobedo Gonzalez, F.
Citation:

Citation: Titaux-Delgado, G.; Carrillo, E.; Mendoza, A.; Martinez-Mayorga, M.; Escobedo-Gonzalez, F.; Cano-Sanchez, P.; Lopez-Vera, E.; Corzo, G.; del Rio Portilla, F.. "Successful refolding and NMR structure of rMagi3: An insecticidal spider toxin rich in disulfide bonds."  Protein Sci. 27, 692-701 (2018).
PubMed: 29247580

Assembly members:

Assembly members:
Mu-hexatoxin-Mg2a, polymer, 46 residues, 5241.110 Da.

Natural source:

Natural source:   Common Name: Spider   Taxonomy ID: 223896   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Macrothele gigas

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mu-hexatoxin-Mg2a: GGCIKWNHSCQTTTLKCCGK CVVCYCHTPWGTNCRCDRTR LFCTED

Data sets:
Data typeCount
1H chemical shifts254

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 46 residues - 5241.110 Da.

1   GLYGLYCYSILELYSTRPASNHISSERCYS
2   GLNTHRTHRTHRLEULYSCYSCYSGLYLYS
3   CYSVALVALCYSTYRCYSHISTHRPROTRP
4   GLYTHRASNCYSARGCYSASPARGTHRARG
5   LEUPHECYSTHRGLUASP

Samples:

sample_1: Magi3 1 ± 0.1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 297 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement

CARA v1.5, Keller and Wuthrich - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - peak picking, structure calculation

NMRPipe v2014, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Bruker AvanceIII 700 MHz