BMRB Entry 30435

Title:
CSP1
Deposition date:
2018-03-12
Original release date:
2018-08-22
Authors:
Yang, Y.
Citation:

Citation: Yang, Y.; Cornilescu, G.; Tal-Gan, Y.. "Structural Characterization of Competence-Stimulating Peptide Analogues Reveals Key Features for ComD1 and ComD2 Receptor Binding in Streptococcus pneumoniae"  Biochemistry 57, 5359-5369 (2018).
PubMed: 30125091

Assembly members:

Assembly members:
Competence-stimulating peptide type 1, polymer, 17 residues, 2248.735 Da.

Natural source:

Natural source:   Common Name: Streptococcus pneumoniae   Taxonomy ID: 1313   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Competence-stimulating peptide type 1: EMRLSKFFRDFILQRKK

Data sets:
Data typeCount
13C chemical shifts28
15N chemical shifts15
1H chemical shifts92

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 17 residues - 2248.735 Da.

1   GLUMETARGLEUSERLYSPHEPHEARGASP
2   PHEILELEUGLNARGLYSLYS

Samples:

sample_1: TFE 20 % v/v; competence stimulating peptide type 1 - CSP1 1.9 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks