BMRB Entry 34028

Title:
Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase
Deposition date:
2016-07-30
Original release date:
2017-12-13
Authors:
Morellet, N.; Taylor, J.; Wieninger, S.; Moriau, S.; Li, X.; Lescop, E.; Mathy, N.; Bischerour, J.; Betermier, M.; Bardiaux, B.; Nilges, M.; Craig, N.; Hickman, A.; Dyda, F.; Guittet, E.
Citation:

Citation: Morellet, Nelly; Li, Xianghong; Wieninger, Silke; Taylor, Jennifer; Bischerour, Julien; Moriau, Severine; Lescop, Ewen; Bardiaux, Benjamin; Mathy, Nathalie; Assrir, Nadine; Betermier, Mireille; Nilges, Michael; Hickman, Alison; Dyda, Fred; Craig, Nancy; Guittet, Eric. "Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase"  Nucleic Acids Res. 46, 2660-2677 (2018).
PubMed: 29385532

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, 5117.271 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Cabbage looper   Taxonomy ID: 7111   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Trichoplusia Ni

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: VMKKRTYCTYCPSKIRRKAN ASCKKCKKVICREHNIDMCQ SCF

Data sets:
Data typeCount
1H chemical shifts303

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_2, 12
3entity_2, 22

Entities:

Entity 1, entity_1 43 residues - 5117.271 Da.

1   VALMETLYSLYSARGTHRTYRCYSTHRTYR
2   CYSPROSERLYSILEARGARGLYSALAASN
3   ALASERCYSLYSLYSCYSLYSLYSVALILE
4   CYSARGGLUHISASNILEASPMETCYSGLN
5   SERCYSPHE

Entity 2, entity_2, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C-ter piggyBac 500 uM; D2O, 100% labelling, 10%; DTT 5 mM; ZnCl2 1500 uM; sodium chloride 200 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

sample_conditions_2: ionic strength: 200 mM; pH: 6.7 pD; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_1isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1
2D DOSYsample_1isotropicsample_conditions_2
2D HMQCsample_1isotropicsample_conditions_2
2D HMQCsample_1isotropicsample_conditions_2

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - structure calculation

CcpNMR v2.3.1, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker AvanceII 800 MHz
  • Bruker AvanceII 950 MHz