BMRB Entry 34045

Title:
Solution NMR structure of the X domain of Peste des Petits Ruminants phosphoprotein
Deposition date:
2016-09-21
Original release date:
2017-10-19
Authors:
Pereira, N.; Piuzzi, M.; Bontems, F.; Eleouet, J.; Sizun, C.
Citation:

Citation: Pereira, N.; Basbous, N.; Piuzzi, M.; Bontems, F.; Eleouet, J.; Sizun, C.. "Solution structure of the X domain of Peste des Petits Ruminants Virus phosphoprotein and interaction with the nucleoprotein"  .

Assembly members:

Assembly members:
entity_1, polymer, 53 residues, 5964.977 Da.

Natural source:

Natural source:   Common Name: Small ruminant morbillivirus   Taxonomy ID: 31604   Superkingdom: Viruses   Kingdom: not available   Genus/species: Morbillivirus Small ruminant morbillivirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts440
15N chemical shifts53
1H chemical shifts746

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 53 residues - 5964.977 Da.

1   GLYSERSERSERARGSERVALILEARGSER
2   ILEILELYSSERSERLYSLEUASNILEASP
3   HISLYSASPTYRLEULEUASPLEULEUASN
4   ASPVALLYSGLYSERLYSASPLEULYSGLU
5   PHEHISLYSMETLEUTHRALAILELEUALA
6   LYSGLNPRO

Samples:

sample_1: PPRV_PXD, [U-13C; U-15N], 200 ± 20 uM; sodium chloride 300 ± 30 mM; sodium phosphate 20 ± 2 mM

sample_2: PPRV_PXD, [U-13C; U-15N], 200 ± 20 uM; sodium chloride 300 ± 30 mM; sodium phosphate 20 ± 2 mM

sample_conditions_1: ionic strength: 300 mM; pH: 7.4; pressure: 1 bar; temperature: 288.0 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_1

Software:

Analysis v2.2.2, CCPN - chemical shift assignment, peak picking

CYANA v3.97, Guntert, Mumenthaler and Wuthrich - structure calculation

TOPSPIN v2.2.2, Bruker Biospin - processing

Talos+, Yang Shen, NIDDK - data analysis

NMR spectrometers:

  • Bruker AvanceIII 800 MHz
  • Bruker AvanceIII 950 MHz