BMRB Entry 34065

Title:
NMR solution structure of Harzianin HK-VI in SDS micelles
Deposition date:
2016-11-17
Original release date:
2018-06-07
Authors:
Kara, S.; Zamora-Carreras, H.; Afonin, S.; Grage, S.; Ulrich, A.; Jimenez, M.
Citation:

Citation: Kara, S.; Afonin, S.; Zamora-Carreras, H.; Bordessa, A.; Doan, V.; Takeshita, N.; Grage, S.; Chaume, G.; Jimenez, M.; Fischer, R.; Bruix, M.; Brigaud, T.; Ulrich, A.; Rebuffat, S.; Goulard, C.; Prigent, Y.; Bodo, B.; Hlimi, S.. "11-mer peptaibol Harzianin HK-VI: conformational and biological analysis"  .

Assembly members:

Assembly members:
entity_1, polymer, 12 residues, 1159.505 Da.

Natural source:

Natural source:   Common Name: Trichoderma pseudokoningii   Taxonomy ID: 317029   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Trichoderma pseudokoningii

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XXNIIXPLLXPX

Data sets:
Data typeCount
13C chemical shifts43
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 12 residues - 1159.505 Da.

1   ACEAIBASNILEILEAIBPROLEULEUAIB
2   PRODCL

Samples:

sample_1: D2O, [U-99% 2H], 10%; DSS, NA, 0.2 mM; H2O 90%; Harzianin HK-VI 1.9 mM; SDS, [U-98% 2H], 185 mM

sample_conditions_1: ionic strength: 0 mM; pH: 7 pH*; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

SPARKY, Goddard - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 600 MHz