BMRB Entry 34129

Title:
Structure of Tau(254-268) bound to F-actin
Deposition date:
2017-05-04
Original release date:
2018-03-09
Authors:
Fontela, Y.; Kadavath, H.; Zweckstetter, M.
Citation:

Citation: Fontela, Y.; Kadavath, H.; Biernat, J.; Riedel, D.; Mandelkow, E.; Zweckstetter, M.. "Structure of Tau(254-268) bound to F-actin"  .

Assembly members:

Assembly members:
entity_1, polymer, 15 residues, 1687.958 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KNVKSKIGSTENLKH

Data sets:
Data typeCount
1H chemical shifts14

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 15 residues - 1687.958 Da.

1   LYSASNVALLYSSERLYSILEGLYSERTHR
2   GLUASNLEULYSHIS

Samples:

sample_1: Tau(254-268) 800 uM; F-actin 27 uM; sodium phosphate 50 mM

sample_2: Tau(254-268) 800 uM; sodium phosphate 50 mM

sample_conditions_1: pH: 6.8; pressure: 1 bar; temperature: 278 K

sample_conditions_2: pH: 6.8; pressure: 1 bar; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker AvanceIII 900 MHz
  • Bruker AvanceIII 800 MHz