BMRB Entry 34273

Title:
NMR structure of temporin B L1FK in SDS micelles
Deposition date:
2018-05-12
Original release date:
2018-06-18
Authors:
Manzo, G.; Mason, J.
Citation:

Citation: Manzo, Giorgia; Ferguson, Philip; Gustilo, V Benjamin; Hind, Charlotte; Clifford, Melanie; Bui, Tam; Drake, Alex; Atkinson, R Andrew; Sutton, J Mark; Batoni, Giovanna; Lorenz, Christian; Phoenix, David; Mason, A James. "Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity"  Sci. Rep. 9, 1385-1385 (2019).
PubMed: 30718667

Assembly members:

Assembly members:
temporinB_L1FK, polymer, 13 residues, 1442.870 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
temporinB_L1FK: FLPIVGLLKSLLK

Data sets:
Data typeCount
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 13 residues - 1442.870 Da.

1   PHELEUPROILEVALGLYLEULEULYSSER
2   LEULEULYS

Samples:

sample_1: deuterated sodium dodecyl sulphate (d-25), U-98% 2H, 100 mM; temporinB_L1FK 2 mM; 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid, 2H, 0.05 % w/w

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

CARA v1.9.1.2, Keller and Wuthrich - chemical shift assignment

DYNAMO, Delaglio and Kuszewski - refinement, structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

NMR spectrometers:

  • Bruker Avance 500 MHz