BMRB Entry 36062

Title:
N-terminal Zinc Finger of Synaptotagmin-like Protein 4
Deposition date:
2017-02-23
Original release date:
2017-10-27
Authors:
Miyamoto, K.
Citation:

Citation: Miyamoto, K.; Nakatani, A.; Saito, K.. "The unique N-terminal zinc finger of Synaptotagmin-like protein 4 reveals FYVE structure"  Protein Sci. 26, 2451-2457 (2017).
PubMed: 28906046

Assembly members:

Assembly members:
Synaptotagmin-like protein 4, polymer, 53 residues, 6040.966 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Synaptotagmin-like protein 4: SDRTCARCQESLGRLSPKTN TCRGCNHLVCRDCRIQESNG TWRCKVCAKEIEL

Data sets:
Data typeCount
1H chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_ZN_12
3entity_ZN_22

Entities:

Entity 1, entity_1 53 residues - 6040.966 Da.

1   SERASPARGTHRCYSALAARGCYSGLNGLU
2   SERLEUGLYARGLEUSERPROLYSTHRASN
3   THRCYSARGGLYCYSASNHISLEUVALCYS
4   ARGASPCYSARGILEGLNGLUSERASNGLY
5   THRTRPARGCYSLYSVALCYSALALYSGLU
6   ILEGLULEU

Entity 2, entity_ZN_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Synaptotagmin-like Protein 4 1 mM; 1,4-DL-dithiothreitol, [U-2H], 1 mM; NaCl 50 mM; Tris-HCl, [U-2H], 20 mM; ZnCl2 50 uM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 250 mM; pH: 6.9; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D NOESYsample_1isotropicsample_conditions_1

Software:

NMRVIEW - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz