BMRB Entry 4012

Title:
Structure of the Acid State of E. coli Ribonuclease HI
Deposition date:
1996-06-21
Original release date:
2001-02-16
Authors:
Dabora, Jonathan; Pelton, Jeffrey; Marqusee, Susan
Citation:

Citation: Dabora, Jonathan; Pelton, Jeffrey; Marqusee, Susan. "Structure of the Acid State of E. coli Ribonuclease H1"  Biochemistry 35, 11951-11958 (1996).
PubMed: 8810899

Assembly members:

Assembly members:
Ribonuclease H1, polymer, 155 residues, 17502 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSM101

Data sets:
Data typeCount
1H chemical shifts276
13C chemical shifts138
15N chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNase H11

Entities:

Entity 1, RNase H1 155 residues - 17502 Da.

1   METLEULYSGLNVALGLUILEPHETHRASP
2   GLYSERALALEUGLYASNPROGLYPROGLY
3   GLYTYRGLYALAILELEUARGTYRARGGLY
4   ARGGLULYSTHRPHESERALAGLYTYRTHR
5   ARGTHRTHRASNASNARGMETGLULEUMET
6   ALAALAILEVALALALEUGLUALALEULYS
7   GLUHISALAGLUVALILELEUSERTHRASP
8   SERGLNTYRVALARGGLNGLYILETHRGLN
9   TRPILEHISASNTRPLYSLYSARGGLYTRP
10   LYSTHRALAASPLYSLYSPROVALLYSASN
11   VALASPLEUTRPGLNARGLEUASPALAALA
12   LEUGLYGLNHISGLNILELYSTRPGLUTRP
13   VALLYSGLYHISALAGLYHISPROGLUASN
14   GLUARGALAASPGLULEUALAARGALAALA
15   ALAMETASNPROTHRLEUGLUASPTHRGLY
16   TYRGLNVALGLUVAL

Samples:

sample_one: Ribonuclease H1, [U-99% 13C; U-99% 15N], 1 – 3 mM

sample_conditions_one: pH: 5.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 1657
PDB
DBJ BAA77885 BAB33633 BAG75734 BAI23570 BAI29084
EMBL CAA23620 CAA27660 CAP74778 CAQ30729 CAQ87812
GB AAA24565 AAB08636 AAC73319 AAG54510 AAN41862
REF NP_308237 NP_414750 NP_706155 WP_000917867 WP_000917875
SP A7ZHV1 A7ZWF6 B1IPU4 B1LHM3 B1XD78
AlphaFold A7ZHV1 A7ZWF6 B1IPU4 B1LHM3 B1XD78

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks