BMRB Entry 4110

Title:
1H, 13C, 15N Resonance Assignment of the 20 KDa Double Stranded RNA Binding Domain of PKR
Deposition date:
1998-02-19
Original release date:
1998-10-14
Authors:
Nanduri, Sambasivarao; Carpick, Bruce; Yang, Yan; Williams, Bryan; Qin, Jun
Citation:

Citation: Nanduri, Sambasivarao; Carpick, Bruce; Yang, Yan; Williams, Bryan; Qin, Jun. "1H, 13C, 15N Resonance Assignment of the 20 KDa Double Stranded RNA Binding Domain of PKR"  J. Biomol. NMR 12, 349-351 (1998).

Assembly members:

Assembly members:
double stranded RNA binding domain of PKR, polymer, 179 residues, 20000 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15B

Data sets:
Data typeCount
13C chemical shifts577
15N chemical shifts176
1H chemical shifts1183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dsRBD-PKR1

Entities:

Entity 1, dsRBD-PKR 179 residues - 20000 Da.

1   GLYSERHISMETGLUMETALAGLYASPLEU
2   SERALAGLYPHEPHEMETGLUGLULEUASN
3   THRARGTYRGLNLYSGLNGLYVALVALLEU
4   LYSTYRGLNGLULEUPROASNSERGLYPRO
5   PROHISASPARGARGPHETHRPHEGLNVAL
6   ILEILEASPGLYARGGLUPHEPROGLUGLY
7   GLUGLYARGSERLYSLYSGLUALALYSASN
8   ALAALAALALYSLEUALAVALGLUILELEU
9   ASNLYSGLULYSLYSALAVALSERPROLEU
10   LEULEUTHRTHRTHRASNSERSERGLUGLY
11   LEUSERMETGLYASNTYRILEGLYLEUILE
12   ASNARGILEALAGLNLYSLYSARGLEUTHR
13   VALASNTYRGLUGLNCYSALASERGLYVAL
14   HISGLYPROGLUGLYPHEHISTYRLYSCYS
15   LYSMETGLYGLNLYSGLUTYRSERILEGLY
16   THRGLYSERTHRLYSGLNGLUALALYSGLN
17   LEUALAALALYSLEUALATYRLEUGLNILE
18   LEUSERGLUGLUTHRGLYSERGLYCYS

Samples:

sample_one: double stranded RNA binding domain of PKR, [U-15N], .9 mM; NaCl 100 mM; sodium phosphate 20 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_two: double stranded RNA binding domain of PKR, [U-15N;U-13C], .9 mM; NaCl 100 mM; sodium phosphate 20 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions_one: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_one

Software:

NMRPipe - used for all data processing

PIPP - analysis of processed spectra

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

PDB
DBJ BAF83344 BAG36554
GB AAA18253 AAA36409 AAC50768 AAF13156 AAH07769
REF NP_001129123 NP_001129124 NP_001138509 NP_001266110 NP_001266614
SP P19525
AlphaFold P19525

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks