BMRB Entry 4171

Title:
Backbone and C beta Assignments of the Anti-gp120 Antibody Fv Fragment Complexed with an Antigenic Peptide
Deposition date:
1998-08-03
Original release date:
2000-03-02
Authors:
Tugarinov, Vitali; Levy, Rina; Dahan-Shokoroy, Adi; Anglister, Jacob
Citation:

Citation: Tugarinov, Vitali; Levy, Rina; Dahan-Shokoroy, Adi; Anglister, Jacob. "Backbone and C beta Assignments of the Anti-gp120 Antibody Fv Fragment Complexed with an Antigenic Peptide"  J. Biomol. NMR 13, 193-194 (1999).

Assembly members:

Assembly members:
light chain, polymer, 112 residues, Formula weight is not available
heavy chain, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTb11

Data typeCount
13C chemical shifts431
15N chemical shifts208
1H chemical shifts449

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FvL1
2FvH2

Entities:

Entity 1, FvL 112 residues - Formula weight is not available

1   ASPILEVALLEUTHRGLNSERPROALASER
2   LEUALAVALSERLEUGLYGLNARGALATHR
3   ILESERCYSLYSALASERGLNSERVALASP
4   TYRASPGLYASPSERTYRMETASNTRPTYR
5   GLNGLNLYSPROGLYGLNPROPROLYSLEU
6   LEUILETYRALAALASERASNLEUGLUSER
7   GLYILEPROALAARGPHESERGLYSERGLY
8   SERARGTHRASPPHETHRLEUASNILEHIS
9   PROVALGLUGLUGLUASPALAALATHRTYR
10   TYRCYSGLNGLNSERASNGLUASPPROPHE
11   THRPHEGLYSERGLYTHRLYSLEUGLUILE
12   LYSARG

Entity 2, FvH 119 residues - Formula weight is not available

1   GLNVALGLNLEUGLNGLNSERGLYALAGLU
2   LEUVALLYSPROGLYALASERVALLYSMET
3   SERCYSLYSALAPHEGLYTYRTHRPHETHR
4   THRTYRPROILEGLUTRPMETLYSGLNASN
5   HISGLYLYSSERLEUGLUTRPILEGLYASN
6   PHEHISPROTYRSERASPASPTHRASNTYR
7   ASNGLULYSPHELYSGLYLYSALALYSLEU
8   THRVALGLULYSSERSERSERTHRVALTYR
9   LEUGLUPHESERARGLEUTHRSERASPASP
10   SERALAVALTYRTYRCYSALAILEHISTYR
11   GLYSERALATYRALAMETASPTYRTRPGLY
12   GLNGLYTHRSERVALTHRVALSERSER

Samples:

sample_one: light chain, [U-95% 13C; U-95% 15N], 0.6 mM; heavy chain, [U-95% 13C; U-95% 15N], 0.6 mM

sample_conditions_one: pH: 7.2; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600.13 MHz
  • Bruker DMX 500.13 MHz

Related Database Links:

PDB
DBJ BAH95819
EMBL CAA75909 CAA80931 CAA80933 CAA80935 CAA80937
GB AAA39088 AAA51113 AAA68538 AAA68560 AAA83114
PIR S42193
SP P01665 P01667
AlphaFold P01665 P01667

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks