BMRB Entry 4278

Title:
Backbone 1H and 15N Chemical Shift Assignments for Long-[L60]-IGF-I, an Insulin-Like Growth Factor 1 Analogue (IGF-I)
Deposition date:
1998-12-07
Original release date:
2002-07-12
Authors:
Laajoki, Leanne; Milner, Steve; Francis, Geoff; Carver, John; Keniry, Max
Citation:

Citation: Laajoki, Leanne; Milner, Steve; Francis, Geoff; Carver, John; Keniry, Max. "1H and 15N Assignments of Long-[L60]-IGF-I, an Insulin-Like Growth Factor I Analogue"  J. Biomol. NMR ., .-. (1999).

Assembly members:

Assembly members:
IGF-I, polymer, 83 residues, 9139 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p[Met1]-pGH(11)-Val-Asn-IGF-I

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts82
1H chemical shifts531
coupling constants59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Long [L60] IGF I1

Entities:

Entity 1, Long [L60] IGF I 83 residues - 9139 Da.

1   METPHEPROALAMETPROLEUSERSERLEU
2   PHEVALASNGLYPROGLUTHRLEUCYSGLY
3   ALAGLULEUVALASPALALEUGLNPHEVAL
4   CYSGLYASPARGGLYPHETYRPHEASNLYS
5   PROTHRGLYTYRGLYSERSERSERARGARG
6   ALAPROGLNTHRGLYILEVALASPGLUCYS
7   CYSPHEARGSERCYSASPLEUARGARGLEU
8   GLUMETLEUCYSALAPROLEULYSPROALA
9   LYSSERALA

Samples:

sample_one: IGF-I, [U-98% 15N], 1.0 mM

sample_conditions_one: pH: 3.0; temperature: 303 K

sample_conditions_two: pH: 3.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_onenot availablenot available
2D 1H-15N HSQC-TOCSYsample_onenot availablenot available
2D 1H-15N HSQC-NOESYsample_onenot availablenot available
3D 15N NOESY-HSQCsample_onenot availablenot available
3D 15N TOCSY-HSQCsample_onenot availablenot available
2D 1H NOESYsample_onenot availablenot available
3D 15N HNHAsample_onenot availablenot available

Software:

VNMR v6.1 - Spectral Processing

XEASY v1.3.10 - Spectral Analysis and Peak Picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15654 2498 4069 4204 4494
PDB
DBJ BAA01200 BAA07897
EMBL CAA27153 CAA28759 CAA35098
GB AAA53535 AAA72204 AAA72455 AAA72579 AAA72763
PIR S22878
PRF 0912651A 2001274B
REF NP_001300784 XP_004418087 XP_004418088 XP_004418089 XP_004418090
SP P33712 Q28933
AlphaFold P33712 Q28933

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks