BMRB Entry 4291

Title:
Solution Structure of Desulfovibrio Vulgaris (Hildenborough) Ferrocytochrome C3: Structural Basis for Functional Cooperativity
Deposition date:
1999-01-07
Original release date:
1999-03-15
Authors:
Messias, A.; Kastrau, D.; Costa, H.; Legall, J.; Turner, D.; Santos, H.; Xavier, A.
Citation:

Citation: Messias, A.; Kastrau, D.; Costa, H.; Legall, J.; Turner, D.; Santos, H.; Xavier, A.. "Solution Structure of Desulfovibrio Vulgaris (Hildenborough) Ferrocytochrome C3: Structural Basis for Functional Cooperativity"  J. Mol. Biol. 281, 719-739 (1998).

Assembly members:

Assembly members:
Cytochrome C3, polymer, 107 residues, 14000 Da.
HEME C, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Desulfovibrio vulgaris   Taxonomy ID: 882   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Desulfovibrio vulgaris

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
Data typeCount
1H chemical shifts668
coupling constants69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome_c3_peptide1
2heme_I2
3heme_II2
4heme_III2
5heme_IV2

Entities:

Entity 1, cytochrome_c3_peptide 107 residues - 14000 Da.

1   ALAPROLYSALAPROALAASPGLYLEULYS
2   METGLUALATHRLYSGLNPROVALVALPHE
3   ASNHISSERTHRHISLYSSERVALLYSCYS
4   GLYASPCYSHISHISPROVALASNGLYLYS
5   GLUASPTYRARGLYSCYSGLYTHRALAGLY
6   CYSHISASPSERMETASPLYSLYSASPLYS
7   SERALALYSGLYTYRTYRHISVALMETHIS
8   ASPLYSASNTHRLYSPHELYSSERCYSVAL
9   GLYCYSHISVALGLUVALALAGLYALAASP
10   ALAALALYSLYSLYSASPLEUTHRGLYCYS
11   LYSLYSSERLYSCYSHISGLU

Entity 2, heme_I - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_one: Cytochrome C3 2 mM; ampicillin 70 uM; kanamicin 50 uM; chloramphenicol 50 uM

sample_conditions_one: pH: 8.5 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D-1H-NOESYsample_onenot availablesample_conditions_one
2D-1H-TOCSYsample_onenot availablesample_conditions_one
2D-1H-COSYsample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
EMBL CAA27847
GB AAS97641 ABM27239 ADP88070
REF WP_010940429 YP_012381
SP P00131
AlphaFold P00131