BMRB Entry 4321

Title:
1H, 13C, and 15N Chemical Shift Assignments for the Catalytic Domain of Tetrahymena GCN5 Histone Acetyltransferase in Complex with CoA
Deposition date:
1999-03-16
Original release date:
2000-12-14
Authors:
Lin, Yingxi; Wagner, Gerhard
Citation:

Citation: Lin, Yingxi; Wagner, Gerhard. "Efficient Side-chain and Backbone Assignment in Large Proteins: Application to tGCN5"  J. Biomol. NMR 15, 227-239 (1999).

Assembly members:

Assembly members:
Tetrahymena histone acetyltransferase, polymer, 166 residues, Formula weight is not available
COENZYME A, non-polymer, 767.534 Da.

Natural source:

Natural source:   Common Name: T. Thermophila   Taxonomy ID: 5911   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Tetrahymena thermophila

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts668
15N chemical shifts175
1H chemical shifts1197

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p551
2CoA2

Entities:

Entity 1, p55 166 residues - Formula weight is not available

1   METLYSGLYLEULEUASPPHEASPILELEU
2   THRASNASPGLYTHRHISARGASNMETLYS
3   LEULEUILEASPLEULYSASNILEPHESER
4   ARGGLNLEUPROLYSMETPROLYSGLUTYR
5   ILEVALLYSLEUVALPHEASPARGHISHIS
6   GLUSERMETVALILELEULYSASNLYSGLN
7   LYSVALILEGLYGLYILECYSPHEARGGLN
8   TYRLYSPROGLNARGPHEALAGLUVALALA
9   PHELEUALAVALTHRALAASNGLUGLNVAL
10   ARGGLYTYRGLYTHRARGLEUMETASNLYS
11   PHELYSASPHISMETGLNLYSGLNASNILE
12   GLUTYRLEULEUTHRTYRALAASPASNPHE
13   ALAILEGLYTYRPHELYSLYSGLNGLYPHE
14   THRLYSGLUHISARGMETPROGLNGLULYS
15   TRPLYSGLYTYRILELYSASPTYRASPGLY
16   GLYTHRLEUMETGLUCYSTYRILEHISPRO
17   TYRVALASPTYRGLYASN

Entity 2, CoA - C21 H36 N7 O16 P3 S - 767.534 Da.

1   COA

Samples:

sample_one: Tetrahymena histone acetyltransferase 0.4 mM; coenzyme A 0.8 mM; sodium phosphate 50 mM; NaCl 100 mM; DTT 0.1 mM; NaN3 0.1 mM; EDTA 0.1 mM

sample_conditions: pH: 6.5 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
GB AAB01099 EAS03638
REF XP_001023869

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks