BMRB Entry 4580

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4580

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Title:
Backbone 1H,13C,and 15N assignments of the anti-dansyl antibody Fv fragment
Deposition date:
2000-02-01
Original release date:
2000-09-25
Authors:
Shindo, Kazuyasu; Masuda, Katsyoshi; Takahashi, Hideo; Arata, Yoji; Shimada, Ichio
Citation:

Citation: Shindo, Kazuyasu; Masuda, Katsyoshi; Takahashi, Hideo; Arata, Yoji; Shimada, Ichio. "Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments of the anti-dansyl antibody Fv fragment"  J. Biomol. NMR 17, 357-358 (2000).

Assembly members:

Assembly members:
VH domain, polymer, 124 residues, 12500 Da.
VL domain, polymer, 113 residues, 12500 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VH domain: EVKLEESGGGLVQPGGSMKL SCATSGFTFSDAWMDWVRQS PEKGLEWVAEIRNKANNHAT YYAESVKGRFTISRDDSKRR VYLQMNTLRAEDTGIYYCTG IYYHYPWFAYWGQGTLVTVS AEPR
VL domain: DVVMTQTPLSLPVSLGNQAS ISCRSSQSLVHSNGNTYLHW YLQKPGQSPKLLIYKVSNRF SGVPDRFSGSGSGTDFTLKI SRVEAEDLGVYFCSQSTHVP FTFGSGTKLEIKR

Data sets:
Data typeCount
1H chemical shifts223
13C chemical shifts217
15N chemical shifts224

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1VH domain1
2VL domain2

Entities:

Entity 1, VH domain 124 residues - 12500 Da.

1   GLUVALLYSLEUGLUGLUSERGLYGLYGLY
2   LEUVALGLNPROGLYGLYSERMETLYSLEU
3   SERCYSALATHRSERGLYPHETHRPHESER
4   ASPALATRPMETASPTRPVALARGGLNSER
5   PROGLULYSGLYLEUGLUTRPVALALAGLU
6   ILEARGASNLYSALAASNASNHISALATHR
7   TYRTYRALAGLUSERVALLYSGLYARGPHE
8   THRILESERARGASPASPSERLYSARGARG
9   VALTYRLEUGLNMETASNTHRLEUARGALA
10   GLUASPTHRGLYILETYRTYRCYSTHRGLY
11   ILETYRTYRHISTYRPROTRPPHEALATYR
12   TRPGLYGLNGLYTHRLEUVALTHRVALSER
13   ALAGLUPROARG

Entity 2, VL domain 113 residues - 12500 Da.

1   ASPVALVALMETTHRGLNTHRPROLEUSER
2   LEUPROVALSERLEUGLYASNGLNALASER
3   ILESERCYSARGSERSERGLNSERLEUVAL
4   HISSERASNGLYASNTHRTYRLEUHISTRP
5   TYRLEUGLNLYSPROGLYGLNSERPROLYS
6   LEULEUILETYRLYSVALSERASNARGPHE
7   SERGLYVALPROASPARGPHESERGLYSER
8   GLYSERGLYTHRASPPHETHRLEULYSILE
9   SERARGVALGLUALAGLUASPLEUGLYVAL
10   TYRPHECYSSERGLNSERTHRHISVALPRO
11   PHETHRPHEGLYSERGLYTHRLYSLEUGLU
12   ILELYSARG

Samples:

sample_1: VH domain, [U-95% 13C; U-95% 15N(except Cys and Trp); [98% 15N]-Cys; [98% 15N]-Trp], 1.2 mM; VL domain, [U-95% 13C; U-95% 15N(except Cys and Trp); [98% 15N]-Cys; [98% 15N]-Trp], 1.2 mM

cond_set_1: pH: 6.0; temperature: 310 K; ionic strength: 0.35 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availablecond_set_1
HNCAsample_1not availablecond_set_1
HN(CO)CAsample_1not availablecond_set_1
15N edited TOCSYsample_1not availablecond_set_1
15N edited NOESYsample_1not availablecond_set_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - processing

UXNMR, Bruker Biospin - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
PIR B30577 PL0257
GenBank AAB22613 AAB28784 AAA39066 AAA85498 AAA39041
EMBL CAB46118
DBJ BAA00052

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks