BMRB Entry 4675

Title:
1H, 13C and 15N resonance assignments of the DNA binding domain of the human forkhead transcription factor AFX
Deposition date:
2000-03-01
Original release date:
2000-07-08
Authors:
Weigelt, Johan; Climent, Isabel; Dahlman-Wright, Karin; Wikstrom, Mats
Citation:

Citation: Weigelt, Johan; Climent, Isabel; Dahlman-Wright, Karin; Wikstrom, Mats. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the DNA binding domain of the human forkhead transcription factor AFX"  J. Biomol. NMR 17, 181-182 (2000).

Assembly members:

Assembly members:
AFX, polymer, 150 residues, 16708 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
15N chemical shifts139
1H chemical shifts529
13C chemical shifts494

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AFX-DBD1

Entities:

Entity 1, AFX-DBD 150 residues - 16708 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   LEUGLUASPPROGLYALAVALTHRGLYPRO
4   ARGLYSGLYGLYSERARGARGASNALATRP
5   GLYASNGLNSERTYRALAGLULEUILESER
6   GLNALAILEGLUSERALAPROGLULYSARG
7   LEUTHRLEUALAGLNILETYRGLUTRPMET
8   VALARGTHRVALPROTYRPHELYSASPLYS
9   GLYASPSERASNSERSERALAGLYTRPLYS
10   ASNSERILEARGHISASNLEUSERLEUHIS
11   SERLYSPHEILELYSVALHISASNGLUALA
12   THRGLYLYSSERSERTRPTRPMETLEUASN
13   PROGLUGLYGLYLYSSERGLYLYSALAPRO
14   ARGARGARGALAALASERMETASPSERSER
15   SERLYSLEULEUARGGLYARGSERLYSALA

Samples:

sample_1: AFX, [U-13C; U-15N], 1.5 mM; Na phosphate 20 mM; NaCl 100 mM; EDTA 1 mM; Pefabloc 1 mM; NaN3 0.02 % w/v

conditions_1: pH: 6.3; temperature: 304 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity Inova 800 MHz

Related Database Links:

PDB
DBJ BAA86199 BAG61928 BAG73163
EMBL CAA63819 CAA72156
GB AAD42108 AAI06762 AAI39276 AAI39305 AAI48035
REF NP_001094747 NP_005929 NP_061259 XP_002720130 XP_002763017
SP P98177 Q9WVH3
TPG DAA12928
AlphaFold P98177 Q9WVH3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks