BMRB Entry 4712

Title:
1H, 15N and 13C Assignments and secondary structure of nFGF
Deposition date:
2000-04-10
Original release date:
2000-07-24
Authors:
Alphonse, Arunkumar; Thallampuranam, Suresh Kumar; Sampath, Srisailam; Kathir, K.; Yu, Chin
Citation:

Citation: Arunkumar, Alphonse; Srisailam, Sampath; Kumar, Thallampuranam; Kathir, K.; Peng, C.; Chen, C.; Chiu, I.; Yu, Chin. "Letter to the Editor: 1H, 13C and 15N chemical shift assignments of the acidic fibroblast growth factor from Notopthalmus viridescens"  J. Biomol. NMR 17, 279-280 (2000).

Assembly members:

Assembly members:
newt acidic FGF, polymer, 132 residues, 15021 Da.
SUCROSE OCTASULFATE, non-polymer, 982.802 Da.

Natural source:

Natural source:   Common Name: newt   Taxonomy ID: 8316   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Notopthalmus viridescens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts530
15N chemical shifts132
1H chemical shifts783

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nFGF1
2sucrose octasulfate2

Entities:

Entity 1, nFGF 132 residues - 15021 Da.

1   GLNLYSPROLYSLEULEUTYRCYSSERASN
2   GLYGLYTYRPHELEUARGILEPHEPROASP
3   GLYLYSVALASPGLYTHRARGASPARGSER
4   ASPPROTYRILEGLNLEUGLNPHETYRALA
5   GLUSERVALGLYGLUVALTYRILELYSSER
6   LEUGLUTHRGLYGLNTYRLEUALAMETASP
7   SERASPGLYGLNLEUTYRALASERGLNSER
8   PROSERGLUGLUCYSLEUPHELEUGLUARG
9   LEUGLUGLUASNASNTYRASNTHRTYRLYS
10   SERLYSVALHISALAASPLYSASPTRPPHE
11   VALGLYILELYSLYSASNGLYLYSTHRLYS
12   PROGLYSERARGTHRHISPHEGLYGLNLYS
13   ALAILELEUPHELEUPROLEUPROVALSER
14   SERASP

Entity 2, sucrose octasulfate - C12 H22 O35 S8 - 982.802 Da.

1   SCR

Samples:

sample_1: newt acidic FGF, [U-95% 13C; U-90% 15N], 1.5 – 2 mM; SUCROSE OCTASULFATE1.5 – 2 mM

Ex-cond: pH: 6.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-EDITED TOCSYsample_1not availableEx-cond
15N-EDITED NOESYsample_1not availableEx-cond
HNCAsample_1not availableEx-cond
HN(CO)CAsample_1not availableEx-cond
13C-EDITED NOESYsample_1not availableEx-cond
HC-CH TOCSYsample_1not availableEx-cond
HNCOsample_1not availableEx-cond
C(CO)NHsample_1not availableEx-cond
HCC0NHsample_1not availableEx-cond
CBCBCONHsample_1not availableEx-cond
CBCANHsample_1not availableEx-cond
HNHAsample_1not availableEx-cond

Software:

XWINNMR-AURELIA v2.1/2.1.5 -

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
SP Q7SIF8
AlphaFold Q7SIF8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks