BMRB Entry 4835

Title:
NMR Structure of the palindromic DNA decamer D(GCGTTAACGC)2
Deposition date:
2000-09-19
Original release date:
2000-12-07
Authors:
Smith, J.; Tsui, V.; Chazin, W.; Case, D.
Citation:

Citation: Smith, Jarrod. "DNA structure by 1H NMR methods: Studies of d(GCGTTAACGC)2"  Thesis The Scripps Research Institute (1999).

Assembly members:

Assembly members:
5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3', polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: .

Entity Sequences (FASTA):

Entity Sequences (FASTA):
5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3': GCGTTAACGC

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GCG10 strand one1
2GCG10 strand two1

Entities:

Entity 1, GCG10 strand one 10 residues - Formula weight is not available

1   DGDCDGDTDTDADADCDGDC

Samples:

sample_1: 5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3' 2 mM; Na3PO4 20 mM; NaCl 50 mM; NaN3 0.1%; D2O 99.9%

sample_2: 5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3' 2 mM; Na3PO4 20 mM; NaCl 50 mM; NaN3 0.1%; D2O 5%; H2O 95%

sample_cond_1: ionic strength: 70 mM; pH: 7; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
Double-Quantumnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1
E-COSYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1

Software:

FELIX v95 - processing

NAB v2.1 - structure calculation

AMBER v5.0 - refinement, iterative matrix relaxation

NMR spectrometers:

  • Bruker AMX 500 MHz