BMRB Entry 4909

Title:
Assignment of 1H, 15N and 13C resonances of the carbohydrate recognition domain of human galectiin-3
Deposition date:
2000-12-05
Original release date:
2001-05-17
Authors:
Umemoto, Kimiko; Leffler, Hakon
Citation:

Citation: Umemoto, Kimiko; Leffler, Hakon. "Letter to the Editor: Assignment of 1H, 15N and 13C resonances of the carbohydrate recognition domain of human galectin-3"  J. Biomol. NMR 20, 91-92 (2001).

Assembly members:

Assembly members:
galectin-3, polymer, 143 residues, Formula weight is not available
LAT, non-polymer, 342.296 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3C

Data sets:
Data typeCount
1H chemical shifts836
13C chemical shifts275
15N chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal fragment of galectin-31
2lactose2

Entities:

Entity 1, C-terminal fragment of galectin-3 143 residues - Formula weight is not available

1   GLYALAPROALAGLYPROLEUILEVALPRO
2   TYRASNLEUPROLEUPROGLYGLYVALVAL
3   PROARGMETLEUILETHRILELEUGLYTHR
4   VALLYSPROASNALAASNARGILEALALEU
5   ASPPHEGLNARGGLYASNASPVALALAPHE
6   HISPHEASNPROARGPHEASNGLUASNASN
7   ARGARGVALILEVALCYSASNTHRLYSLEU
8   ASPASNASNTRPGLYARGGLUGLUARGGLN
9   SERVALPHEPROPHEGLUSERGLYLYSPRO
10   PHELYSILEGLNVALLEUVALGLUPROASP
11   HISPHELYSVALALAVALASNASPALAHIS
12   LEULEUGLNTYRASNHISARGVALLYSLYS
13   LEUASNGLUILESERLYSLEUGLYILESER
14   GLYASPILEASPLEUTHRSERALASERTYR
15   THRMETILE

Entity 2, lactose - C12 H22 O11 - 342.296 Da.

1   LAT

Samples:

sample_1: galectin-3, [U-99% 13C; U-99% 15N], 0.5 mM; LACTOSE 10 mM; potassium phosphate 5 mM

Ex-cond_1: pH: 7.4; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
HCC-TOCSYsample_1not availableEx-cond_1
CN-NOESY-HSQCsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian UNITYplus 500 MHz

Related Database Links:

BMRB 15705 19491 7422
PDB
DBJ BAA22164 BAD92628 BAG37435 BAI46476
EMBL CAD61918 CAG33178 CAG46894
GB AAA35607 AAA36163 AAA88086 AAB26229 AAB86584
REF NP_002297 XP_001148424 XP_002824813 XP_003831735 XP_004055252
SP P17931
AlphaFold P17931

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks