BMRB Entry 4932

Title:
NMR Structure of wheat nonspecific lipid transfer protein
Deposition date:
2000-12-21
Original release date:
2001-01-09
Authors:
Caille, Anita; Vovelle, Francoise; Sy, Denise
Citation:

Citation: Caille, Anita; Vovelle, Francoise; Sy, Denise. "1H Assigned Chemical Shifts for Wheat ns-LTP."  .

Assembly members:

Assembly members:
non specific lipid transfer protein, polymer, 90 residues, 9000 Da.

Natural source:

Natural source:   Common Name: Wheat   Taxonomy ID: 4565   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Triticum aestivum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
non specific lipid transfer protein: IDCGHVDSLVRPCLSYVQGG PGPSGQCCDGVKNLHNQARS QSDRQSACNCLKGIARGIHN LNEDNARSIPPKCGVNLPYT ISLNIDCSRV

Data sets:
Data typeCount
1H chemical shifts597
coupling constants69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nonspecific lipid transfer protein1

Entities:

Entity 1, nonspecific lipid transfer protein 90 residues - 9000 Da.

1   ILEASPCYSGLYHISVALASPSERLEUVAL
2   ARGPROCYSLEUSERTYRVALGLNGLYGLY
3   PROGLYPROSERGLYGLNCYSCYSASPGLY
4   VALLYSASNLEUHISASNGLNALAARGSER
5   GLNSERASPARGGLNSERALACYSASNCYS
6   LEULYSGLYILEALAARGGLYILEHISASN
7   LEUASNGLUASPASNALAARGSERILEPRO
8   PROLYSCYSGLYVALASNLEUPROTYRTHR
9   ILESERLEUASNILEASPCYSSERARGVAL

Samples:

sample_1: non specific lipid transfer protein 4 mM; acetate buffer 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.3; temperature: 308 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-NOESY 60-ms mixing timesample_1not availablesample_cond_1

Software:

UXNMR - data processing

XEASY - data analysis

INFIT - J-coupling analysis

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 16415 2065 4383
PDB
EMBL CAA45210 CAH69187
GB AAB22334 AAN75627
PIR S22528
PRF 1814270A
SP P24296
AlphaFold P24296