BMRB Entry 5014

Title:
Sequence Specific Resonance Assignment of the Central Domain of Cardiac Myosin Binding Protein C (MyBP-C)
Deposition date:
2001-05-21
Original release date:
2005-02-16
Authors:
Idowu, Seraphina; Gautel, Mathias; Pfuhl, Mark
Citation:

Citation: Idowu, Seraphina; Gautel, Mathias; Pfuhl, Mark. "Sequence Specific Resonance Assignment of the Central Domain of Cardiac Myosin Binding Protein C (MyBP-C)"  J. Biomol. NMR 22, 199-200 (2002).
PubMed: 11883787

Assembly members:

Assembly members:
domain 5 of cardiac myosin binding protein C, polymer, 130 residues, 14243.0 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts745
13C chemical shifts480
15N chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cC51

Entities:

Entity 1, cC5 130 residues - 14243.0 Da.

1   ARGGLNGLUPROPROLYSILEHISLEUASP
2   CYSPROGLYARGILEPROASPTHRILEVAL
3   VALVALALAGLYASNLYSLEUARGLEUASP
4   VALPROILESERGLYASPPROALAPROTHR
5   VALILETRPGLNLYSALAILETHRGLNGLY
6   ASNLYSALAPROALAARGPROALAPROASP
7   ALAPROGLUASPTHRGLYASPSERASPGLU
8   TRPVALPHEASPLYSLYSLEULEUCYSGLU
9   THRGLUGLYARGVALARGVALGLUTHRTHR
10   LYSASPARGSERILEPHETHRVALGLUGLY
11   ALAGLULYSGLUASPGLUGLYVALTYRTHR
12   VALTHRVALLYSASNPROVALGLYGLUASP
13   GLNVALASNLEUTHRVALLYSVALILEASP

Samples:

sample_1: domain 5 of cardiac myosin binding protein C, [U-95% 13C; U-95% 15N], 0.7 – 1.3 mM; NaN3 0.02%; EDTA 2 mM; DTT 4 mM

sample_2: domain 5 of cardiac myosin binding protein C, [U-95% 15N], 1.4 mM; NaN3 0.02%; EDTA 2 mM; DTT 4 mM

standard_conditions: pH: 7.0; temperature: 298 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
1H 15N NOESYnot availablenot availablenot available
1H 15N TOCSYnot availablenot availablenot available
1H 13C NOESYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HSQCnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available

Software:

nmrPipe v1.8 - spectral processing

ANSIG v3.3 - spectral analysis

NMR spectrometers:

  • Varian UnityPlus 600 MHz
  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
DBJ BAF84258 BAG60177
EMBL CAA58882 CAA71216
GB AAC04620 AAI36544 AAI36547 AAI42686 AAI51212
REF NP_000247 XP_002808117 XP_002821860 XP_003278987 XP_003815230
SP Q14896
AlphaFold Q14896

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks