BMRB Entry 5352

Title:
1H, 13C and 15N resonance assignment of Bacillus agaradhaerens xylanase
Deposition date:
2002-04-25
Original release date:
2002-09-25
Authors:
Betz, Marco; Wienk, Hans; Lohr, Frank; Ruterjans, Heinz
Citation:

Citation: Betz, Marco; Lohr, Frank; Wienk, Hans; Ruterjans, Heinz. "Letter to the Editor: 1H, 13C and 15N resonance assignment of Bacillus agaradhaerens family 11 xylanase"  J. Biomol. NMR 23, 333-334 (2002).

Assembly members:

Assembly members:
xylanase B.agaradhaerens, polymer, 207 residues, 23142 Da.

Natural source:

Natural source:   Common Name: Bacillus agaradhaerens   Taxonomy ID: 76935   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus agaradhaerens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts1157
13C chemical shifts847
15N chemical shifts211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1xylanase, monomer1

Entities:

Entity 1, xylanase, monomer 207 residues - 23142 Da.

1   METILEVALTHRASPASNSERILEGLYASN
2   HISASPGLYTYRASPTYRGLUPHETRPLYS
3   ASPSERGLYGLYSERGLYTHRMETILELEU
4   ASNHISGLYGLYTHRPHESERALAGLNTRP
5   ASNASNVALASNASNILELEUPHEARGLYS
6   GLYLYSLYSPHEASNGLUTHRGLNTHRHIS
7   GLNGLNVALGLYASNMETSERILEASNTYR
8   GLYALAASNPHEGLNPROASNGLYASNALA
9   TYRLEUCYSVALTYRGLYTRPTHRVALASP
10   PROLEUVALGLUTYRTYRILEVALASPSER
11   TRPGLYASNTRPARGPROPROGLYALATHR
12   PROLYSGLYTHRILETHRVALASPGLYGLY
13   THRTYRASPILETYRGLUTHRLEUARGVAL
14   ASNGLNPROSERILELYSGLYILEALATHR
15   PHELYSGLNTYRTRPSERVALARGARGSER
16   LYSARGTHRSERGLYTHRILESERVALSER
17   ASNHISPHEARGALATRPGLUASNLEUGLY
18   METASNMETGLYLYSMETTYRGLUVALALA
19   LEUTHRVALGLUGLYTYRGLNSERSERGLY
20   SERALAASNVALTYRSERASNTHRLEUARG
21   ILEASNGLYASNPROLEUSER

Samples:

sample_1: xylanase B.agaradhaerens, [U-98% 13C; U-98% 15N], 1.4 mM

Ex-cond_1: pH: 5.3; temperature: 303 K; ionic strength: 0.01 M

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1not availableEx-cond_1
(HCA)CO(CA)NHsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CC(CO)NH-TOCSYsample_1not availableEx-cond_1
HBHACONHsample_1not availableEx-cond_1
H(CC)(CO)NH-TOCSYsample_1not availableEx-cond_1
HCD(CG)CBsample_1not availableEx-cond_1
HN(CDCG)CBsample_1not availableEx-cond_1
CBCACO(CA)HAsample_1not availableEx-cond_1

Software:

Pronto v19990506 - assignment

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 15908 16583
PDB
GB AEP40122 AEP40123 AEP40124

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks