BMRB Entry 5524

Title:
Resonance Assignment and Topology of a Clostridial Repetitive Oligopeptide (CROP) Region of Toxin A from Clostridium Difficile
Deposition date:
2002-09-10
Original release date:
2003-01-14
Authors:
Rappas, Mathieu; Lim, Jenson; Simpson, Peter; Prasannan, Sunil; Fairweather, Neil; Matthews, Stephen
Citation:

Citation: Rappas, Mathieu; Lim, Jenson; Simpson, Peter; Prasannan, Sunil; Fairweather, Neil; Matthews, Stephen. "Letter to the Editor: Resonance Assignment and Topology of a Clostridial Repetitive Oligopeptide (CROP) Region of Toxin A from Clostridium difficile"  J. Biomol. NMR 25, 83-84 (2003).

Assembly members:

Assembly members:
Toxin A sub-unit, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Cl. difficile   Taxonomy ID: 1496   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Clostridium difficile

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts484
15N chemical shifts131
1H chemical shifts766

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Toxin A C-terminal fragment1

Entities:

Entity 1, Toxin A C-terminal fragment 116 residues - Formula weight is not available

1   METGLYALAASNGLYTYRLYSTHRILEASP
2   ASNLYSASNPHETYRPHEARGASNGLYLEU
3   PROGLNILEGLYVALPHELYSGLYSERASN
4   GLYPHEGLUTYRPHEALAPROALAASNTHR
5   ASPALAASNASNILEGLUGLYGLNALAILE
6   ARGTYRGLNASNARGPHELEUHISLEULEU
7   GLYLYSILETYRTYRPHEGLYASNASNSER
8   LYSALAVALTHRGLYTRPGLNTHRILEASN
9   GLYLYSVALTYRTYRPHEMETPROASPTHR
10   ALAMETALAALAALAGLYGLYLEUPHEGLU
11   ILEASPGLYVALILETYRPHEPHEGLYVAL
12   ASPGLYVALLYSALAPRO

Samples:

sample_1: Toxin A sub-unit, [U-13C; U-15N], 0.25 – 0.5 mM; Sodium acetate 10 mM

Ex-cond_1: ionic strength: 0.01 mM; pH: 5.5; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
The NOESY-HSQC used simultaneous detection of C13/N15. The spectrometer has asample_1not availableEx-cond_1
cryoprobe.sample_1not availableEx-cond_1

Software:

AURELIA v2.8.11 - Standard "strip-based" assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

GB EQF33976 EQI36199
REF WP_021386796

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks