BMRB Entry 5619

Title:
Resonance assignments for the methionine sulfoxide reductase B from Bacillus subtilis, Northeast Structural Genomics Consortium target SR10
Deposition date:
2002-12-09
Original release date:
2002-12-27
Authors:
Zheng, Deyou; Cort, John; Chiang, Yiwen; Kennedy, Michael; Montelione, Gaetano
Citation:

Citation: Zheng, Deyou; Cort, John; Chiang, Yiwen; Acton, Thomas; Kennedy, Michael; Montelione, Gaetano. "Letter to the Editor: 1H, 13C and 15N resonance assignments for methionine sulfoxide reductase B from Bacillus subtilis"  J. Biomol. NMR 27, 183-184 (2003).

Assembly members:

Assembly members:
methionine sulfoxide reductase msrB, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts259
13C chemical shifts447
15N chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Methionine sulfoxide reductase1

Entities:

Entity 1, Methionine sulfoxide reductase 151 residues - Formula weight is not available

1   METALATYRASNLYSGLUGLULYSILELYS
2   SERLEUASNARGMETGLNTYRGLUVALTHR
3   GLNASNASNGLYTHRGLUPROPROPHEGLN
4   ASNGLUTYRTRPASPHISLYSGLUGLUGLY
5   LEUTYRVALASPILEVALSERGLYLYSPRO
6   LEUPHETHRSERLYSASPLYSPHEASPSER
7   GLNCYSGLYTRPPROSERPHETHRLYSPRO
8   ILEGLUGLUGLUVALGLUGLULYSLEUASP
9   THRSERHISGLYMETILEARGTHRGLUVAL
10   ARGSERARGTHRALAASPSERHISLEUGLY
11   HISVALPHEASNASPGLYPROGLYPROASN
12   GLYLEUARGTYRCYSILEASNSERALAALA
13   LEUARGPHEVALPROLYSHISLYSLEULYS
14   GLUGLUGLYTYRGLUSERTYRLEUHISLEU
15   PHEASNLYSLEUGLUHISHISHISHISHIS
16   HIS

Samples:

sample_1: methionine sulfoxide reductase msrB, [U-13C; U-15N; U-2H] with Val, Leu, Ile(delta) methyl protonated, 1.0 mM

Ex-cond_1: pH: 6.5 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
H(CCCO)NH-TOCSYnot availablenot availablenot available
(H)CC(CO)NH-TOCSYnot availablenot availablenot available

Software:

FELIX - process

AutoAssign v1.9 - automated backbone assignments

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

BMRB 17008
PDB
DBJ BAI85662 BAM52650 BAM58225 GAK79338
EMBL CAB14086 CCU58667 CEI57380 CEJ77805
GB AAA96648 ADV92894 AEP91184 AFQ58114 AGA23472
REF NP_390051 WP_003230813 WP_014477116 WP_014480061 WP_021481498
SP P54155
AlphaFold P54155

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks