BMRB Entry 5628

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5628

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Title:
1H, 15N and 13C resonance assignments of the highly conserved 19kDa C-terminal domain from human Elongation Factor 1Bgamma
Deposition date:
2002-12-17
Original release date:
2003-04-30
Authors:
Vanwetswinkel, Sophie; Kriek, Jan; Andersen, Gregers; Dijk, Jan; Canters, Gerard; Siegal, Gregg
Citation:

Citation: Vanwetswinkel, Sophie; Kriek, Jan; Andersen, Gregers; Dijk, Jan; Siegal, Gregg. "Letter to the Editor: 1H, 15N and 13C resonance assignments of the highly conserved 19kDa C-terminal domain from human Elongation Factor 1Bgamma"  J. Biomol. NMR 26, 189-190 (2003).

Assembly members:

Assembly members:
C-terminal domain [276-437] of the human EF1Bgamma subunit from elongation factor 1, polymer, 162 residues, 19045 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal domain [276-437] of the human EF1Bgamma subunit from elongation factor 1: AKDPFAHLPKSTFALDEFKR KYSNEDTLSVALPYFWEHFD KDGWSLWYSEYRFPEELTQT FMSCNLITGMFQRLDKLRKN AFASVILFGTNNSSSISGVW VFRGQELAFPLSPDWQVDYE SYTWRKLDPGSEETQTLVRE YFSWEGAFQHVGKAFNQGKI FK

Data sets:
Data typeCount
1H chemical shifts1156
13C chemical shifts561
15N chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1eEF1Bgamma[276-437]1

Entities:

Entity 1, eEF1Bgamma[276-437] 162 residues - 19045 Da.

1   ALALYSASPPROPHEALAHISLEUPROLYS
2   SERTHRPHEALALEUASPGLUPHELYSARG
3   LYSTYRSERASNGLUASPTHRLEUSERVAL
4   ALALEUPROTYRPHETRPGLUHISPHEASP
5   LYSASPGLYTRPSERLEUTRPTYRSERGLU
6   TYRARGPHEPROGLUGLULEUTHRGLNTHR
7   PHEMETSERCYSASNLEUILETHRGLYMET
8   PHEGLNARGLEUASPLYSLEUARGLYSASN
9   ALAPHEALASERVALILELEUPHEGLYTHR
10   ASNASNSERSERSERILESERGLYVALTRP
11   VALPHEARGGLYGLNGLULEUALAPHEPRO
12   LEUSERPROASPTRPGLNVALASPTYRGLU
13   SERTYRTHRTRPARGLYSLEUASPPROGLY
14   SERGLUGLUTHRGLNTHRLEUVALARGGLU
15   TYRPHESERTRPGLUGLYALAPHEGLNHIS
16   VALGLYLYSALAPHEASNGLNGLYLYSILE
17   PHELYS

Samples:

sample_1: C-terminal domain [276-437] of the human EF1Bgamma subunit from elongation factor 1, [U-15N], 1.0 mM; Tris 20 mM; potassium chloride 75 mM; DTT 1 mM; sodium azide 0.02%

sample_2: C-terminal domain [276-437] of the human EF1Bgamma subunit from elongation factor 1, [U-15N; U-13C], 1.0 mM; Tris 20 mM; potassium chloride 75 mM; DTT 1 mM; sodium azide 0.02%

sample_3: C-terminal domain [276-437] of the human EF1Bgamma subunit from elongation factor 1, [U-10% 13C], 1.0 mM; Tris 20 mM; potassium chloride 75 mM; DTT 1 mM; sodium azide 0.02%

cond_1: pH: 7.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker Avance 750 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
DBJ BAB25320 BAC34356 BAC38351 BAE00947 BAE26482
EMBL CAA45089 CAA77630 CAG28553
GB AAC18414 AAF69604 AAH00384 AAH06509 AAH06520
REF NP_001004223 NP_001035577 NP_001075254 NP_001182509 NP_001289353
SP A2Q127 P26641 Q29387 Q3SZV3 Q4R7H5
TPG DAA13740
AlphaFold A2Q127 P26641 Q29387 Q3SZV3 Q4R7H5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks