BMRB Entry 5682

Title:
Solution Structure of 30S Ribosomal Protein S27E from Archaeoglobus Fulgidus: RS27_ARCFU: a novel fold
Deposition date:
2003-01-31
Original release date:
2004-06-30
Authors:
Herve Du Penhoat, C.; Atreya, H.; Shen, Y.; Liu, G.; Acton, T.; Xiao, R.; Montelione, G.; Szyperski, T.
Citation:

Citation: Herve Du Penhoat, C.; Atreya, H.; Shen, Y.; Liu, G.; Acton, T.; Xiao, R.; Dang, J.; Murray, D.; Montelione, G.; Szyperski, T.. "Solution Structure of 30S Ribosomal Protein S27E from Archaeoglobus Fulgidus: RS27_ARCFU: a novel fold"  Protein Sci. 13, 1407-1416 (2004).
PubMed: 15096641

Assembly members:

Assembly members:
S27 ribosomal protein, polymer, 66 residues, 7578 Da.

Natural source:

Natural source:   Common Name: Archaeoglobus fulgidus   Taxonomy ID: 2234   Superkingdom: Archaea   Kingdom: Fungi   Genus/species: Archaeoglobus fulgidus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S27 ribosomal protein: MHSRFVKVKCPDCEHEQVIF DHPSTIVKCIICGRTVAEPT GGKGNIKAEIIEYVDQIELE HHHHHH

Data sets:
Data typeCount
1H chemical shifts408
13C chemical shifts239
15N chemical shifts58
coupling constants33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
130S RIBOSOMAL PROTEIN S27E1

Entities:

Entity 1, 30S RIBOSOMAL PROTEIN S27E 66 residues - 7578 Da.

1   METHISSERARGPHEVALLYSVALLYSCYS
2   PROASPCYSGLUHISGLUGLNVALILEPHE
3   ASPHISPROSERTHRILEVALLYSCYSILE
4   ILECYSGLYARGTHRVALALAGLUPROTHR
5   GLYGLYLYSGLYASNILELYSALAGLUILE
6   ILEGLUTYRVALASPGLNILEGLULEUGLU
7   HISHISHISHISHISHIS

Samples:

Sample_1: S27 ribosomal protein, [U-100% 13C; U-100% 15N], 0.8 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; D2O 5%; H2O 95%

Sample2: S27 ribosomal protein, [U-5% 13C; U-90% 15N], 0.8 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; CaCl2 5 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_cond_1: pH: 6.5 na; temperature: 298 K; pressure: 1.0 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-SEPARATED NOESYnot availablenot availablesample_cond_1
3D 15N-SEPARATED NOESYnot availablenot availablesample_cond_1
3D HNCACBnot availablenot availablesample_cond_1
3D HCCH-COSY RDnot availablenot availablesample_cond_1
3D HABCAB(CO)NH RDnot availablenot availablesample_cond_1
3D HNHAnot availablenot availablesample_cond_1
3D HNCOnot availablenot availablesample_cond_1
2D [1H-1H] NOESYnot availablenot availablesample_cond_1
2D C13HSQCnot availablenot availablesample_cond_1
Constant time gChsqcnot availablenot availablesample_cond_1
non-Constant time gChsqcnot availablenot availablesample_cond_1
3D HNCOnot availablenot availablesample_cond_1

Software:

CYANA v1.05 - refinement

DYANA v1.5 - structure solution

MOLMOL v1.0 - structure solution

XEASY v1.3.3 - structure solution

NMR spectrometers:

  • VARIAN UNITYPlus 500 MHz
  • VARIAN UNITYPlus 600 MHz

Related Database Links:

PDB
GB AAB89911 AIG98214
REF NP_070163 WP_010878831
SP O28935
AlphaFold O28935

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks