BMRB Entry 5689

Title:
1H, 13C, and 15N backbone assignments of the pheromone binding protein from the silk moth Antheraea polyphemus (ApolPBP)
Deposition date:
2003-02-07
Original release date:
2005-10-17
Authors:
Mohanty, Smita; Zubkov, Sergey; Campos-Olivas, Ramon
Citation:

Citation: Mohanty, Smita; Zubkov, Sergey; Campos-Olivas, Ramon. "Letter to the Editor: 1H, 13C and 15N Backbone Assignments of the Pheromone Binding Protein from the Silk Moth Antheraea polyphemus (ApolPBP)"  J. Biomol. NMR 27, 393-394 (2003).
PubMed: 14512737

Assembly members:

Assembly members:
Pheromone-binding protein from Antheraea polyphemus, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Polyphemus moth   Taxonomy ID: 7120   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Antheraea polyphemus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHN1+

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts548
15N chemical shifts147
1H chemical shifts939

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ApolPBP1

Entities:

Entity 1, ApolPBP 142 residues - Formula weight is not available

1   SERPROGLUILEMETLYSASNLEUSERASN
2   ASNPHEGLYLYSALAMETASPGLNCYSLYS
3   ASPGLULEUSERLEUPROASPSERVALVAL
4   ALAASPLEUTYRASNPHETRPLYSASPASP
5   TYRVALMETTHRASPARGLEUALAGLYCYS
6   ALAILEASNCYSLEUALATHRLYSLEUASP
7   VALVALASPPROASPGLYASNLEUHISHIS
8   GLYASNALALYSASPPHEALAMETLYSHIS
9   GLYALAASPGLUTHRMETALAGLNGLNLEU
10   VALASPILEILEHISGLYCYSGLULYSSER
11   ALAPROPROASNASPASPLYSCYSMETLYS
12   THRILEASPVALALAMETCYSPHELYSLYS
13   GLUILEHISLYSLEUASNTRPVALPROASN
14   METASPLEUVALILEGLYGLUVALLEUALA
15   GLUVAL

Samples:

sample_1: Pheromone-binding protein from Antheraea polyphemus, [U-99% 13C; U-99% 15N], 1.0 mM

backbone_conditions: pH: 6.33; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HMQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D CC(CO)NHnot availablenot availablenot available
3D HCACOnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D 1H-15N-1H NOESYnot availablenot availablenot available
3D 1H-13C-1H NOESYnot availablenot availablenot available

Software:

NMRPipe v2.1 -

NMRView v5.0.4 -

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15256
PDB
EMBL CAA35592
GenBank AAB49502
PRF 1515253A
SWISS-PROT P20797

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks