BMRB Entry 5793

Title:
Backbone 1H, 15N, 13C and side chain 13Cb assignment of a 42 kDa RecR homodimer
Deposition date:
2003-05-07
Original release date:
2004-02-11
Authors:
Honda, Masayoshi; Ito, Yutaka
Citation:

Citation: Honda, Masayoshi; Rajesh, Sundaresan; Nietlispach, Daniel; Mikawa, Tsutomu; Shibata, Takehiko; Ito, Yutaka. "Letter to the Editor: Backbone 1H, 13C, 15N assignments of a 42 kDa RecR homodimer"  J. Biomol. NMR 28, 199-200 (2004).

Assembly members:

Assembly members:
Thermus thermophilus HB8 RecR, polymer, 194 residues, 21192.5 Da.

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts549
15N chemical shifts177
1H chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RecR subunit 11
2RecR subunit 21

Entities:

Entity 1, RecR subunit 1 194 residues - 21192.5 Da.

1   METARGTYRPROGLUSERLEULEULYSLEU
2   THRARGALALEUSERARGLEUPROGLYILE
3   GLYPROLYSTHRALAGLNARGLEUALALEU
4   HISLEUALAPHEHISLYSGLUGLUALAGLU
5   ALALEUALAGLUALALEUGLUGLYILELYS
6   ARGVALARGALACYSARGGLUCYSGLYASN
7   LEUALAGLUGLYGLULEUCYSPROILECYS
8   GLNASPGLUASPARGASPARGSERLEULEU
9   ALAVALVALGLUSERVALALAASPLEUTYR
10   ALALEUGLUARGSERGLYGLUPHEARGGLY
11   LEUTYRHISVALLEUGLYGLYALALEUASN
12   PROLEUGLUGLYILEGLYPROLYSGLULEU
13   ASNLEUGLUGLYLEUPHEARGARGLEUGLU
14   GLYVALGLUGLUVALVALLEUALATHRSER
15   METTHRVALGLUGLYGLUALATHRALALEU
16   TYRLEUALAGLUGLULEULYSLYSARGGLY
17   VALARGVALTHRARGPROALATYRGLYLEU
18   PROVALGLYGLYSERLEUGLUTYRALAASP
19   GLUVALTHRLEUGLYARGALALEUGLUGLY
20   ARGARGPROVAL

Samples:

sample_1: Thermus thermophilus HB8 RecR, [U-98% 2H; U-98% 13C; U-98% 15N], 0.6 mM

cond_set_1: pH: 7.2 na; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HN(CA)CBnot availablenot availablenot available
3D HN(COCA)CBnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D HNCOnot availablenot availablenot available

Software:

AZARA v2.7 - data processing

ANSIG for OpenGL v1.0.5 - peak assignments

NMR spectrometers:

  • BRUKER DRX 600 MHz

Related Database Links:

DBJ BAD71423
GB AAS81578 AEG34015 AFH38354 EIA38760
REF WP_008633233 WP_011228798 WP_014629118 YP_005205 YP_005641142
SP Q5SHY0 Q72I96
AlphaFold Q5SHY0 Q72I96

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks