BMRB Entry 5857

Title:
NMR assignment of human Sip(7-50)
Deposition date:
2003-07-03
Original release date:
2006-06-26
Authors:
Santelli, E.; Liddington, R.; Preece, N.
Citation:

Citation: Santelli, E.; Leone, M.; Li, C.; Fukushima, T.; Preece, N.; Olson, A.; Ely, K.; Reed, J.; Pellecchia, M.; Liddington, R.; Matsuzawa, S.. "Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex"  J. Biol. Chem. 280, 34278-34287 (2005).
PubMed: 16085652

Assembly members:

Assembly members:
GSH-SIP(short), polymer, 51 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GSH-SIP(short): GSHMAASEELQKDLEEVKVL LEKATRKRVRDALTAEKSKI ETEIKNKMQQK

Data sets:
Data typeCount
13C chemical shifts175
15N chemical shifts43
1H chemical shifts226
coupling constants6

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GSH-SIP7-50 subunit11
2GSH-SIP7-50 subunit21

Entities:

Entity 1, GSH-SIP7-50 subunit1 51 residues - Formula weight is not available

1   GLYSERHISMETALAALASERGLUGLULEU
2   GLNLYSASPLEUGLUGLUVALLYSVALLEU
3   LEUGLULYSALATHRARGLYSARGVALARG
4   ASPALALEUTHRALAGLULYSSERLYSILE
5   GLUTHRGLUILELYSASNLYSMETGLNGLN
6   LYS

Samples:

sample_1: GSH-SIP(short), [U-15N; U-13C], 1.0 mM

sample_cond_1: ionic strength: 1.0 M; pH: 5.1; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
watergate/presat H1-H1 NOESYnot availablenot availablenot available
N15-HSQCnot availablenot availablenot available
N15-TOCSYHSQCnot availablenot availablenot available
N15-NOESYHSQCnot availablenot availablenot available
C13-HSQCnot availablenot availablenot available
C(CO)NH/C(CC-TOCSY-CO)N-NHnot availablenot availablenot available
H(CCO)NH/H(CC-TOCSY-CO)N-NHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

VNMR v6.1C - collection

FELIX v2000 - processing, spectral display

NMR spectrometers:

  • Varian UNITYplus 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks