BMRB Entry 5896

Title:
Unusual Ca2+-calmodulin binding interactions of the microtubule-associated protein F-STOP
Deposition date:
2003-08-05
Original release date:
2004-02-11
Authors:
Bouvier, Denis; Vanhaverbeke, Cecile; Simorre, Jean-Pierre; Gans, Pierre
Citation:

Citation: Bouvier, Denis; Vanhaverbeke, Cecile; Simorre, Jean-Pierre; Arlaud, Gerard; Bally, Isabelle; Forge, Vincent; Margolis, Robert; Gans, Pierre; Kleman, Jean-Philippe. "Unusual Ca(2+)-calmodulin Binding Interactions of the Microtubule-associated Protein F-STOP"  Biochemistry 42, 11484-11493 (2003).
PubMed: 14516200

Assembly members:

Assembly members:
calmodulin, polymer, 163 residues, 18522 Da.
F-STOP Mc module, polymer, 23 residues, 2739 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source:   Production method: chemical synthesis

Data sets:
Data typeCount
1H chemical shifts152
13C chemical shifts153
15N chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin1
2F-STOP Mc module2
3CALCIUM (II) ION3

Entities:

Entity 1, calmodulin 163 residues - 18522 Da.

1   METSERHISHISHISHISHISHISSERMET
2   ASPILEGLUPHEMETALAASPGLNLEUTHR
3   ASPGLUGLNILEALAGLUPHELYSGLUALA
4   PHESERLEUPHEASPLYSASPGLYASPGLY
5   THRILETHRTHRLYSGLULEUGLYTHRVAL
6   METARGSERLEUGLYGLNASNPROTHRGLU
7   ALAGLULEUGLNASPMETILEASNGLUVAL
8   ASPALAASPGLYASNGLYTHRILEASPPHE
9   PROGLUPHELEUASNLEUMETALAARGLYS
10   METLYSASPTHRASPSERGLUGLUGLULEU
11   LYSGLUALAPHEARGVALPHEASPLYSASP
12   GLYASNGLYPHEILESERALAALAGLULEU
13   ARGHISVALMETTHRASNLEUGLYGLULYS
14   LEUTHRASPGLUGLUVALASPGLUMETILE
15   ARGGLUALAASPVALASPGLYASPGLYGLN
16   VALASNTYRGLUGLUPHEVALGLNVALMET
17   METALALYS

Entity 2, F-STOP Mc module 23 residues - 2739 Da.

1   GLNARGASPTHRARGARGLYSALAGLYPRO
2   ALATRPMETVALTHRARGTHRGLUGLYHIS
3   GLUGLULYS

Entity 3, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: calmodulin, [U-97% 13C; U-98% 15N], 0.7 mM; F-STOP Mc module 0.7 mM

Condition_1: pH: 7.7; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableCondition_1
HNCAsample_1not availableCondition_1

Software:

FELIX v2000 - raw spectral data processing, peak picking

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

GB ERE78236
REF NP_058900.1 NP_058900.1 NP_058900.1 NP_058900.1 NP_001036820.2
PRF 2208500A
GenBank EDM18414.1 EDM18414.1 EDM18414.1 EDM18414.1 EDM18415.1
EMBL CAA05555.1 CAA05555.1 CAA05555.1 CAA05555.1 CAA05555.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks