BMRB Entry 6048

Title:
NEW STRUCTURAL FAMILY OF AN ANTIMICROBIAL PEPTIDE DERIVED FROM BOVINE HEMOGLOBIN
Deposition date:
2003-12-15
Original release date:
2008-07-16
Authors:
Sforca, Mauricio; Figueredo, Rita; Machado, Alessandra; Oyama, Sergio; Miranda, Maria; Miranda, Antonio; Daffre, Sirlei; Spisni, Alberto; Pertinhez, Thelma
Citation:

Citation: Sforca, Mauricio; Machado, Alessandra; Figueredo, Rita; Oyama, Sergio; Silva, F.; Miranda, Antonio; Daffre, Sirlei; Miranda, Maria; Spisni, Alberto; Pertinhez, Thelma. "The Micelle-Bound Structure of an Antimicrobial Peptide Derived from the alpha-Chain of Bovine Hemoglobin Isolated from the Tick Boophilus microplus."  Biochemistry 44, 6440-6451 (2005).
PubMed: 15850378

Assembly members:

Assembly members:
Hemoglobin fragment, polymer, 30 residues, 3205 Da.

Natural source:

Natural source:   Common Name: southern cattle tick   Taxonomy ID: 6941   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Boophilus microplus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hemoglobin fragment: FLSFPTTKTYFPHFDLSHGS AQVKGHGAKX

Data sets:
Data typeCount
1H chemical shifts178
coupling constants23

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hb3361a monomer1

Entities:

Entity 1, Hb3361a monomer 30 residues - 3205 Da.

1   PHELEUSERPHEPROTHRTHRLYSTHRTYR
2   PHEPROHISPHEASPLEUSERHISGLYSER
3   ALAGLNVALLYSGLYHISGLYALALYSNH2

Samples:

sample_1: Hemoglobin fragment 1 mM; SDS 200 mM

Hb3361a_condition: pH: 4.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D Noesysample_1not availableHb3361a_condition
2D Tocsysample_1not availableHb3361a_condition
2D DQFCOSYsample_1not availableHb3361a_condition

Software:

FELIX - semi-automated peak assignments

NMRPIPE-NMRVIEW - semi-automated peak assignments

DYANA - simulated annealing

DISCOVER - energy minimization

INSIGHTII - structure vizualization

PROCHECK-NMR - statistical analysis

CNS - statistical analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 1101 16891 16898 25 2707 2709 2868 3442 5809 6360 6413 6414 907
PDB
DBJ BAD97112 BAJ20323
EMBL CAA23748 CAA23749 CAA23750 CAA23752 CAA23774
GB AAA30909 AAA30910 AAA36844 AAA52631 AAB22948
PIR A24693 HAMQR
PRF 0907233A 1004268B 1004272A 1004272B 1509322A
REF NP_000508 NP_000549 NP_001036091 NP_001036092 NP_001038189
SP P01923 P01924 P01926 P01928 P01929
TPG DAA15749
AlphaFold P01923 P01924 P01926 P01928 P01929