BMRB Entry 6080

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6080

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C resonance assignments of the human protein tyrosine phosphatase PRL-1

Deposition date:

2004-01-14

Original release date:

2008-09-18

Authors:

Laurence, Jennifer; Hallenga, Klaas; Stauffacher, Cynthia

Citation:

Citation: Laurence, Jennifer; Hallenga, Klaas; Stauffacher, Cynthia. "Letter to the Editor: 1H, 15N, 13C resonance assignments of the human protein tyrosine phosphatase PRL-1" J. Biomol. NMR 29, 417-418 (2004).
PubMed: 15213447

Assembly members:

Assembly members:
PRL-1, polymer, 173 residues, 19815 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PRL-1: MARMNRPAPVEVTYKNMRFL ITHNPTNATLNKFIEELKKY GVTTIVRVCEATYDTTLVEK EGIHVLDWPFDDGAPPSNQI VDDWLSLVKIKFREEPGCCI AVHCVAGLGRAPVLVALALI EGGMKYEDAVQFIRQKRRGA FNSKQLLYLEKYRPKMRLRF KDSNGHRNNCCIQ

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	314
15N chemical shifts	129
1H chemical shifts	673

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PRL-1 monomer	1

Entities:

Entity 1, PRL-1 monomer 173 residues - 19815 Da.

1

MET

ALA

ARG

MET

ASN

ARG

PRO

ALA

PRO

VAL

2

GLU

VAL

THR

TYR

LYS

ASN

MET

ARG

PHE

LEU

3

ILE

THR

HIS

ASN

PRO

THR

ASN

ALA

THR

LEU

4

ASN

LYS

PHE

ILE

GLU

LEU

LYS

TYR

5

GLY

VAL

THR

ILE

VAL

ARG

VAL

CYS

GLU

6

ALA

THR

TYR

ASP

THR

LEU

VAL

GLU

LYS

7

GLU

GLY

ILE

HIS

VAL

LEU

ASP

TRP

PRO

PHE

8

ASP

GLY

ALA

PRO

SER

ASN

GLN

ILE

9

VAL

ASP

TRP

LEU

SER

LEU

VAL

LYS

ILE

10

LYS

PHE

ARG

GLU

PRO

GLY

CYS

ILE

11

ALA

VAL

HIS

CYS

VAL

ALA

GLY

LEU

GLY

ARG

12

ALA

PRO

VAL

LEU

VAL

ALA

LEU

ALA

LEU

ILE

13

GLU

GLY

MET

LYS

TYR

GLU

ASP

ALA

VAL

14

GLN

PHE

ILE

ARG

GLN

LYS

ARG

GLY

ALA

15

PHE

ASN

SER

LYS

GLN

LEU

TYR

LEU

GLU

16

LYS

TYR

ARG

PRO

LYS

MET

ARG

LEU

ARG

PHE

17

LYS

ASP

SER

ASN

GLY

HIS

ARG

ASN

CYS

18

CYS

ILE

GLN

Samples:

sample_1: PRL-1, [U-95% 13C; U-98% 15N], 1.3 – 2.0 mM

sample_2: PRL-1, [U-98% 15N], 1.5 – 2.0 mM

sample_3: PRL-1, [1H methyl only, 2H U-98%; U-95% 13C; U-98% 15N], 2.0 mM

Ex-cond_1: pH: 6.5; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
CBCACONH	not available	not available	not available
HNCACB	not available	not available	not available
CCONH	not available	not available	not available
HCCONH	not available	not available	not available
CCCONH	not available	not available	not available
HNCO	not available	not available	not available
1H-15N NOESY	not available	not available	not available
1H-13C NOESY	not available	not available	not available
15N-HSQC	not available	not available	not available
13C-HSQC	not available	not available	not available

Software:

SPARKY v3 -

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks