BMRB Entry 6252

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6252

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Title:
1H, 13C, 15N Resonance Assignments of the EscJ Protein, a Structural Component of the Type III Secretion System of Enteropathogenic E. coli (EPEC)
Deposition date:
2004-06-25
Original release date:
2004-12-15
Authors:
Prasannan, Sunil; Simpson, Peter; Wilson, Rebecca; Frankel, Gad; Matthews, Stephen
Citation:

Citation: Prasannan, Sunil; Simpson, Peter; Wilson, Rebecca; Crepin, Valerie; Frankel, Gad; Matthews, Stephen. "Letter to the Editor: 1H, 13C, 15N Resonance Assignments of the EscJ Protein, a Structural Component of the Type III Secretion System of Enteropathogenic E. coli (EPEC)"  J. Biomol. NMR 30, 387-388 (2004).

Assembly members:

Assembly members:
Type III Secretion System component EscJ, polymer, 170 residues, 18544 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Type III Secretion System component EscJ: KEQLYTGLTEKEANQMQALL LSNDVNVSKEMDKSGNMTLS VEKEDFVRAITILNNNGFPK KKFADIEVIFPPSQLVASPS QENAKINYLKEQDIERLLSK IPGVIDCSVSLNVNNNESQP SSAAVLVISSPEVNLAPSVI QIKNLVKNSVDDLKLENISV VIKSSSGQDG

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts291
13C chemical shifts446
15N chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EscJ monomer1

Entities:

Entity 1, EscJ monomer 170 residues - 18544 Da.

1   LYSGLUGLNLEUTYRTHRGLYLEUTHRGLU
2   LYSGLUALAASNGLNMETGLNALALEULEU
3   LEUSERASNASPVALASNVALSERLYSGLU
4   METASPLYSSERGLYASNMETTHRLEUSER
5   VALGLULYSGLUASPPHEVALARGALAILE
6   THRILELEUASNASNASNGLYPHEPROLYS
7   LYSLYSPHEALAASPILEGLUVALILEPHE
8   PROPROSERGLNLEUVALALASERPROSER
9   GLNGLUASNALALYSILEASNTYRLEULYS
10   GLUGLNASPILEGLUARGLEULEUSERLYS
11   ILEPROGLYVALILEASPCYSSERVALSER
12   LEUASNVALASNASNASNGLUSERGLNPRO
13   SERSERALAALAVALLEUVALILESERSER
14   PROGLUVALASNLEUALAPROSERVALILE
15   GLNILELYSASNLEUVALLYSASNSERVAL
16   ASPASPLEULYSLEUGLUASNILESERVAL
17   VALILELYSSERSERSERGLYGLNASPGLY

Samples:

Sample_1: Type III Secretion System component EscJ, [U-13C; U-15N], mM; sodium chloride 30 mM; Imidazole 50 mM; sodium phosphate 50 mM

sample_2: Type III Secretion System component EscJ, [U-13C; U-15N], mM; sodium chloride 30 mM; Imidazole 30 mM; sodium phosphate 50 mM

sample_3: Type III Secretion System component EscJ, [U-15N]; [13C]-Val, Ile, (non-backbone) Leu, mM; sodium chloride 30 mM; Imidazole 50 mM; sodium phosphate 50 mM

conditions_1: pH: 7.2 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

XWIN-NMR - Spectral Processing

AURELIA - Peak-picking, Sequential Assigment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAB37996 BAG66748 BAI28408 BAI32346 BAI37542
EMBL CAC81857 CAG17525 CAI43879 CAR47976 CAR48046
GB AAC31518 AAC38379 AAG58837 AAK26710 AAL30751
REF NP_290273 NP_312600 WP_000408508 WP_000716186 WP_000716187

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks