BMRB Entry 6313

Title:
1H,13C,and 15N NMR assignments of the Bombyx mori Pheromone-binding Protein fragment BmPBP(1-128) at pH 6.5.
Deposition date:
2004-09-03
Original release date:
2005-02-08
Authors:
Damberger, Fred; Michel, Erich; Wuthrich, Kurt
Citation:

Citation: Michel, Erich; Damberger, Fred; Chen, Angela; Ishida, Yuko; Leal, Walter; Wuthrich, Kurt. "Letter to the Editor: Assignments for the Bombyx mori pheromone-binding protein fragment BmPBP(1-128) at pH 6.5"  J. Biomol. NMR 31, 65-65 (2005).

Assembly members:

Assembly members:
Bombyx mori Pheromone-binding protein, polymer, 128 residues, 14467 Da.

Natural source:

Natural source:   Common Name: silkworm moth   Taxonomy ID: 7091   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bombyx mori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts902
13C chemical shifts555
15N chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BmPBP1

Entities:

Entity 1, BmPBP 128 residues - 14467 Da.

1   SERGLNGLUVALMETLYSASNLEUSERLEU
2   ASNPHEGLYLYSALALEUASPGLUCYSLYS
3   LYSGLUMETTHRLEUTHRASPALAILEASN
4   GLUASPPHETYRASNPHETRPLYSGLUGLY
5   TYRGLUILELYSASNARGGLUTHRGLYCYS
6   ALAILEMETCYSLEUSERTHRLYSLEUASN
7   METLEUASPPROGLUGLYASNLEUHISHIS
8   GLYASNALAMETGLUPHEALALYSLYSHIS
9   GLYALAASPGLUTHRMETALAGLNGLNLEU
10   ILEASPILEVALHISGLYCYSGLULYSSER
11   THRPROALAASNASPASPLYSCYSILETRP
12   THRLEUGLYVALALATHRCYSPHELYSALA
13   GLUILEHISLYSLEUASNTRPALA

Samples:

sample_1: Bombyx mori Pheromone-binding protein, [U-99% 13C; U-98% 15N], 1.1 mM; potassium phosphate buffer 50 mM; H2O 95%; D2O 5%; NaN3 0.2%

Ex-cond_1: pH: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HN(CA)COsample_1not availableEx-cond_1
(H)CCH-COSYsample_1not availableEx-cond_1
15N-resolved [1H,1H]-TOCSYsample_1not availableEx-cond_1
(HB)CB(CGCD)HDsample_1not availableEx-cond_1
15N-resolved [1H,1H]-NOESYsample_1not availableEx-cond_1
13C(aliphatic)-resolved [1H,1H]-NOESYsample_1not availableEx-cond_1
13C(aromatic)-resolved [1H,1H]-NOESYsample_1not availableEx-cond_1

Software:

CARA v1.1 - computer-aided resonance assignment

ATNOS - automated peak-picking, integration

CANDID - automated NOE crosspeak assignment for structure calculation

DYANA - automated structure calculation using NMR constraints

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 900 MHz

Related Database Links:

BMRB 4849
PDB
EMBL CAA64443
GB ACT34881 AGR44744 AGR44745 AGR44746 AGR44747
REF NP_001037494
SP P34174
AlphaFold P34174 P34174 P34174

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks