BMRB Entry 6346

Title:
1H, 15N and 13C Resonance Assignments and Secondary Structure Determination Reveal that the Minimal Rac1 GTPase Binding Domain of Plexin-B1 Has a Ubiquitin Fold
Deposition date:
2004-10-10
Original release date:
2005-07-25
Authors:
Tong, Yufeng; Buck, Matthias
Citation:

Citation: Tong, Yufeng; Buck, Matthias. "Letter to the Editor: 1H, 15N and 13C Resonance assignments and secondary structure determination reveal that the minimal Rac1 GTPase binding domain of plexin-B1 has a ubiquitin fold"  J. Biomol. NMR 31, 369-370 (2005).
PubMed: 15929008

Assembly members:

Assembly members:
plexin-B1 RBD, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts517
15N chemical shifts123
1H chemical shifts840

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1plexin-B1 RBD-W1830F1

Entities:

Entity 1, plexin-B1 RBD-W1830F 122 residues - Formula weight is not available

1   LYSLYSASPVALGLUTYRARGPROLEUTHR
2   LEUASNALALEULEUALAVALGLYPROGLY
3   ALAGLYGLUALAGLNGLYVALPROVALLYS
4   VALLEUASPCYSASPTHRILESERGLNALA
5   LYSGLULYSMETLEUASPGLNLEUTYRLYS
6   GLYVALPROLEUTHRGLNARGPROASPPRO
7   ARGTHRLEUASPVALGLUTRPARGSERGLY
8   VALALAGLYHISLEUILELEUSERASPGLU
9   ASPVALTHRSERGLUVALGLNGLYLEUPHE
10   ARGARGLEUASNTHRLEUGLNHISTYRLYS
11   VALPROASPGLYALATHRVALALALEUVAL
12   PROCYSLEUTHRLYSHISVALLEUARGGLU
13   ASNGLN

Samples:

sample_1: plexin-B1 RBD, [U-13C; U-15N], 1.2 mM

sample_2: plexin-B1 RBD, [U-13C], 2 mM

condition_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C CT-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
H(CCCO)NHnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available
HCCH-TOSYnot availablenot availablenot available
RD-HBCB(CGCD)HDnot availablenot availablenot available
1H-TOCSY-relayed RD-HCH-COSYnot availablenot availablenot available
NOESY-15N-HSQCnot availablenot availablenot available
NOESY-13C-HSQCnot availablenot availablenot available

Software:

NMRView v5.2.2 - Spectral Analysis

NMRPipe v2.3 - Data processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker ICE 600 MHz

Related Database Links:

PDB
DBJ BAA23703 BAG10332 BAG52423 BAG52582
EMBL CAB56222 CAB57277
GB AAI46794 EAW64864 EAW64865 EAW64866
REF NP_001123554 NP_002664 XP_003257054 XP_003926345 XP_004034120
SP O43157
AlphaFold O43157

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks