BMRB Entry 6399

Title:
1H, 13C and 15N resonance assignments for the 24 kDa TPR domain in p67phox
Deposition date:
2004-11-23
Original release date:
2005-07-26
Authors:
Yoshida, Shinichi; Ogura, Kenji; Yokochi, Masashi; Yuzawa, Satoru; Horiuchi, Masataka; Morioka, Hiroshi; Sumimoto, Hideki; Inagaki, Fuyuhiko
Citation:

Citation: Yoshida, Shinichi; Ogura, Kenji; Yokochi, Masashi; Yuzawa, Satoru; Horiuchi, Masataka; Morioka, Hiroshi; Sumimoto, Hideki; Inagaki, Fuyuhiko. "1H, 13C and 15N resonance assignments of the backbone and methyl groups of the 24 kDa tetratricopeptide repeat domain in p67phox"  J. Biomol. NMR 32, 176-176 (2005).

Assembly members:

Assembly members:
TPR, polymer, 208 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPROEX-HTb

Data sets:
Data typeCount
13C chemical shifts675
1H chemical shifts457
15N chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TPR domain in p67phox1

Entities:

Entity 1, TPR domain in p67phox 208 residues - Formula weight is not available

1   GLYALAMETGLYSERMETSERLEUVALGLU
2   ALAILESERLEUTRPASNGLUGLYVALLEU
3   ALAALAASPLYSLYSASPTRPLYSGLYALA
4   LEUASPALAPHESERALAVALGLNASPPRO
5   HISSERARGILECYSPHEASNILEGLYCYS
6   METTYRTHRILELEULYSASNMETTHRGLU
7   ALAGLULYSALAPHETHRARGSERILEASN
8   ARGASPLYSHISLEUALAVALALATYRPHE
9   GLNARGGLYMETLEUTYRTYRGLNTHRGLU
10   LYSTYRASPLEUALAILELYSASPLEULYS
11   GLUALALEUILEGLNLEUARGGLYASNGLN
12   LEUILEASPTYRLYSILELEUGLYLEUGLN
13   PHELYSLEUPHEALACYSGLUVALLEUTYR
14   ASNILEALAPHEMETTYRALALYSLYSGLU
15   GLUTRPLYSLYSALAGLUGLUGLNLEUALA
16   LEUALATHRSERMETLYSSERGLUPROARG
17   HISSERLYSILEASPLYSALAMETGLUCYS
18   VALTRPLYSGLNLYSLEUTYRGLUPROVAL
19   VALILEPROVALGLYLYSLEUPHEARGPRO
20   ASNGLUARGGLNVALALAGLNLEUALALYS
21   LYSASPTYRLEUGLYLYSALATHR

Samples:

sample_1: TPR, [U-80%(fractional) 2H; U-98% 13C; U-98% 15N], 0.8 mM; Na-Pi 50 mM; NaCl 150 mM; DTT 1 mM; MgCl2 10 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_2: TPR, [NA-Val, Leu and Ile(d1) methyl; U-98% 2H; U-98% 13C; U-98% 15N], 0.7 mM; Na-Pi 50 mM; NaCl 150 mM; DTT 1 mM; MgCl2 10 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availableconditions_1
2D 1H-13C CT-HSQCnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HN(CA)COnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HN(CO)CAnot availablenot availableconditions_1
HN(CA)CBnot availablenot availableconditions_1
HN(COCA)CBnot availablenot availableconditions_1
C(CO)NHnot availablenot availableconditions_1
H(CCO)NHnot availablenot availableconditions_1
H(C)CH-TOCSYnot availablenot availableconditions_1

Software:

NMRPipe/NMRDraw - processing, peak-pick

Olivia, Yokochi Masashi - assignment works

NMR spectrometers:

  • Varian Unity-Plus or AS 600 MHz
  • Varian AS 500 MHz

Related Database Links:

PDB
DBJ BAD92884 BAG35548 BAJ20290
EMBL CAH92976
GB AAA36379 AAB60320 AAH01606 AAM89263 AAP36107
REF NP_000424 NP_001121123 NP_001127613 XP_001114105 XP_003264493
SP P19878
AlphaFold P19878

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks