BMRB Entry 6503

Title:
1H, 13C, and 15N complete chemical shift assignments for the apo v-Src SH2 domain
Deposition date:
2005-02-16
Original release date:
2005-10-24
Authors:
Taylor, Jonathan; Williams, Mark; Ababou, Abdessamad; Ladbury, John
Citation:

Citation: Taylor, Jonathan; Fawaz, Radwan; Ababou, Abdessamad; Williams, Mark; Ladbury, John. "NMR Assignment of the Apo and Peptide-bound SH2 Domain from the Rous Sarcoma Viral Protein Src"  J. Biomol. NMR 32, 339-339 (2005).
PubMed: 16211495

Assembly members:

Assembly members:
v-Src SH2 domain, polymer, 106 residues, 12168 Da.

Natural source:

Natural source:   Common Name: Rous Sarcoma Virus   Taxonomy ID: 11886   Superkingdom: viruses   Kingdom: not available   Genus/species: Alpharetrovirus Rous Sarcoma Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
13C chemical shifts456
15N chemical shifts120
1H chemical shifts702

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1v-Src SH21

Entities:

Entity 1, v-Src SH2 106 residues - 12168 Da.

1   GLNALAGLUGLUTRPTYRPHEGLYLYSILE
2   THRARGARGGLUSERGLUARGLEULEULEU
3   ASNPROGLUASNPROARGGLYTHRPHELEU
4   VALARGGLUSERGLUTHRTHRLYSGLYALA
5   TYRCYSLEUSERVALSERASPPHEASPASN
6   ALALYSGLYLEUASNVALLYSHISTYRLYS
7   ILEARGLYSLEUASPSERGLYGLYPHETYR
8   ILETHRSERARGTHRGLNPHESERSERLEU
9   GLNGLNLEUVALALATYRTYRSERLYSHIS
10   ALAASPGLYLEUCYSHISARGLEUTHRASN
11   VALCYSPROTHRSERLYS

Samples:

Sample_1: v-Src SH2 domain, [U-95% 13C; U-90% 15N], 0.5 mM; NaCl 50 mM

Sample_2: v-Src SH2 domain, [U-90% 15N], 0.5 mM

Conditions: ionic strength: 0.05 M; pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOWNY-HSQCnot availablenot availablenot available
1H-13C NOESY-HSQCnot availablenot availablenot available
1H-13C HCCH-TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
CBHDnot availablenot availablenot available

Software:

ANSIG v3.3 - Manual peak assignment

NMR spectrometers:

  • Varian UNITY-INOVA 800 MHz
  • Varian UNITY-INOVA 600 MHz
  • Varian UNITY-INOVA 500 MHz

Related Database Links:

PDB
DBJ BAA01500 BAE26865 BAI47379
EMBL CAA23696 CAA24495 CAA26485 CAA32012 CAA36156
GB AAA40135 AAA42563 AAA42565 AAA42573 AAA42581
PRF 0903255A
REF NP_001020566 NP_001104274 NP_001248263 NP_005408 NP_033297
SP P00523 P00524 P00525 P05480 P12931
TPG DAA23281
AlphaFold P00523 P00524 P00525 P05480 P12931

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks