BMRB Entry 6565

Title:
Assignment of 1H, 13C and 15N resonances of barnacle cement protein Mrcp-20k
Deposition date:
2005-03-22
Original release date:
2005-09-01
Authors:
Suzuki, Rintaro; Mori, Youichi; Kamino, Kei; Yamazaki, Toshimasa
Citation:

Citation: Suzuki, Rintaro; Mori, Youichi; Kamino, Kei; Yamazaki, Toshimasa. "NMR Assignment of the Barnacle Cement Protein Mrcp-20k"  J. Biomol. NMR 32, 257-257 (2005).
PubMed: 16132826

Assembly members:

Assembly members:
Mrcp20k, polymer, 186 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Acorn barnacle   Taxonomy ID: 6680   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Megabalanus rosa

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts619
15N chemical shifts172
1H chemical shifts908

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mrcp20k1

Entities:

Entity 1, Mrcp20k 186 residues - Formula weight is not available

1   ALAMETALAHISGLUGLUASPGLYVALCYS
2   ASNSERASNALAPROCYSTYRHISCYSASP
3   ALAASNGLYGLUASNCYSSERCYSASNCYS
4   GLULEUPHEASPCYSGLUALALYSLYSPRO
5   ASPGLYSERTYRALAHISPROCYSARGARG
6   CYSASPALAASNASNILECYSLYSCYSSER
7   CYSTHRALAILEPROCYSASNGLUASPHIS
8   PROCYSHISHISCYSHISGLUGLUASPASP
9   GLYASPTHRHISCYSHISCYSSERCYSGLU
10   HISSERHISASPHISHISASPASPASPTHR
11   HISGLYGLUCYSTHRLYSLYSALAPROCYS
12   TRPARGCYSGLUTYRASNALAASPLEULYS
13   HISASPVALCYSGLYCYSGLUCYSSERLYS
14   LEUPROCYSASNASPGLUHISPROCYSTYR
15   ARGLYSGLUGLYGLYVALVALSERCYSASP
16   CYSLYSTHRILETHRCYSASNGLUASPHIS
17   PROCYSTYRHISSERTYRGLUGLUASPGLY
18   VALTHRLYSSERASPCYSASPCYSGLUHIS
19   SERPROGLYPROSERGLU

Samples:

sample_1: Mrcp20k, [U-13C; U-15N], 7 mM; Sodium Phosphate 10 mM; D2O 10%

sample_2: Mrcp20k, [U-13C; U-15N], 7 mM; Sodium Phosphate 10 mM; D2O 99.8%

condition_1: pH*: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availablecondition_1
3D HNCOnot availablenot availablecondition_1
3D HNCACBnot availablenot availablecondition_1
3D CBCA(CO)NHnot availablenot availablecondition_1
3D HNCAnot availablenot availablecondition_1
3D 15N-separated NOESY-HSQCnot availablenot availablecondition_1
3D 13C/15N-separated NOESY-HSQCnot availablenot availablecondition_1
1H13C HSQCnot availablenot availablecondition_1
3D HCCH-TOCSYnot availablenot availablecondition_1
4D 13C/13C-separated HSQC-NOESY-HSQCnot availablenot availablecondition_1

Software:

NMRPipe v2.3 -

SPARKY v3.110 -

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

DBJ BAB18762

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks