BMRB Entry 6626

Name: 'Complete 1H, 13C, and 15N Chemical Shift Assignments for plant Tom20 mitochondrial import receptor cytosolic domain (isoform 3) from Arabidopsis thaliana'
Published: 2005-12-14

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6626

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

'Complete 1H, 13C, and 15N Chemical Shift Assignments for plant Tom20 mitochondrial import receptor cytosolic domain (isoform 3) from Arabidopsis thaliana'

Deposition date:

2005-05-12

Original release date:

2005-12-14

Authors:

Perry, Andrew; Hulett, Joanne; Lithgow, Trevor; Gooley, Paul

Citation:

Citation: Perry, Andrew; Hulett, Joanne; Lithgow, Trevor; Gooley, Paul. "1H, 13C and 15N Resonance Assignments of the Cytosolic Domain of Tom20 from Arabidopsis thaliana" J. Biomol. NMR 33, 198-198 (2005).

Assembly members:

Assembly members:
Plant Tom20 receptor, polymer, 158 residues, 17954.8 Da.

Natural source:

Natural source: Common Name: Mouse-ear cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Vector: pGEX-6-3P-AtTom20-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Plant Tom20 receptor: GPLGSMDTETEFDRILLFEQ IRQDAENTYKSNPLDADNLT RWGGVLLELSQFHSISDAKQ MIQEAITKFEEALLIDPKKD EAVWCIGNAYTSFAFLTPDE TEAKHNFDLATQFFQQAVDE QPDNTHYLKSLEMTAKAPQL HAEAYKQGLGGSHHHHHH

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	620
15N chemical shifts	159
1H chemical shifts	1039

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Plant Tom20	1

Entities:

Entity 1, Plant Tom20 158 residues - 17954.8 Da.

1

GLY

PRO

LEU

GLY

SER

MET

ASP

THR

GLU

THR

2

GLU

PHE

ASP

ARG

ILE

LEU

PHE

GLU

GLN

3

ILE

ARG

GLN

ASP

ALA

GLU

ASN

THR

TYR

LYS

4

SER

ASN

PRO

LEU

ASP

ALA

ASP

ASN

LEU

THR

5

ARG

TRP

GLY

VAL

LEU

GLU

LEU

SER

6

GLN

PHE

HIS

SER

ILE

SER

ASP

ALA

LYS

GLN

7

MET

ILE

GLN

GLU

ALA

ILE

THR

LYS

PHE

GLU

8

GLU

ALA

LEU

ILE

ASP

PRO

LYS

ASP

9

GLU

ALA

VAL

TRP

CYS

ILE

GLY

ASN

ALA

TYR

10

THR

SER

PHE

ALA

PHE

LEU

THR

PRO

ASP

GLU

11

THR

GLU

ALA

LYS

HIS

ASN

PHE

ASP

LEU

ALA

12

THR

GLN

PHE

GLN

ALA

VAL

ASP

GLU

13

GLN

PRO

ASP

ASN

THR

HIS

TYR

LEU

LYS

SER

14

LEU

GLU

MET

THR

ALA

LYS

ALA

PRO

GLN

LEU

15

HIS

ALA

GLU

ALA

TYR

LYS

GLN

GLY

LEU

GLY

16

GLY

SER

HIS

Samples:

sample_1: Plant Tom20 receptor, [U-95% 13C; U-95% 15N], 0.8 ± 0.08 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02%; dithiothreitol 1 mM; ethylenediaminetetraacetic acid 1 mM; phenylmethylsulphonylfluoride 1 mM; deuterium dioxide%

sample_2: Plant Tom20 receptor, [U-95% 13C; U-95% 15N], 0.8 ± 0.08 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02%; dithiothreitol 1 mM; ethylenediaminetetraacetic acid 1 mM; phenylmethylsulphonylfluoride 1 mM; deuterium dioxide%

sample_3: Plant Tom20 receptor, [U-95% 15N], 0.8 ± 0.08 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02%; dithiothreitol 1 mM; ethylenediaminetetraacetic acid 1 mM; phenylmethylsulphonylfluoride 1 mM; deuterium dioxide%

conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC	sample_1	not available	conditions_1
13C_1H-HSQC	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
C(CO)NH-TOCSY	sample_1	not available	conditions_1
H(CCO)NH-TOCSY	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HCCH-TOCSY	sample_1	not available	conditions_1
HCCH-COSY	sample_1	not available	conditions_1
HN(CA)CO	sample_1	not available	conditions_1
13C HSQC-NOESY	sample_1	not available	conditions_1
13C NOESY-HSQC (aromatic region)	sample_1	not available	conditions_1
[13C,1H]-HSQC (aromatic region)	sample_1	not available	conditions_1
15N NOESY-HSQC	sample_1	not available	conditions_1

Software:

NMRPipe v1.1, Frank Delaglio - spectra processing

SPARKY v3.110, T. D. Goddard and D. G. Kneller - spectral assignment

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 500 MHz

PDB	1ZU2
DBJ	BAB01089
EMBL	CAC14430
GB	AAK64184 AAL85142 AAM64598 AEE77264
REF	NP_189344
SP	P82874
AlphaFold	P82874